描述
HW-55F
形式
beads
包装
bottle of 500 mL
参数
3 bar max. pressure
技术
HPLC: suitable
LPLC: suitable
基质
hydroxylated methacrylic polymer
基质活性基团
hydroxylated methacrylate phase
粒径
45 μm
孔径
1000-200,000 Da MW range
工作pH值
2-13
分离技术
size exclusion (SEC)
一般描述
Toyopearl HW-55 resin is a hydroxylated methacrylic polymer bead for size exclusion chromatography of proteins between 1,000-700,000 Da. It also is the base bead for all Toyopearl "-550" designated products.
应用
TOYOPEARL® media fractionate mixtures of proteins and other large molecular weight compounds over a wide size range. Applications include separating RNA from protein, resolving oligosaccharides by degree of polymerization, and isolating agglutinin while maintaining a high hemagglutination titer.
产品规格
Clean in place with 0.5 M NaOH or 0.1 M HCl.
外形
Shipped in 20% (v/v) ethanol.
法律信息
Toyopearl is a registered trademark of Tosoh Corporation
警示用语:
Danger
危险声明
危险分类
Flam. Liq. 2
储存分类代码
3 - Flammable liquids
WGK
WGK 3
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
Carbohydrate research, 347(1), 161-163 (2011-11-22)
A method is developed for the preparation of D-rhamnose from an O-polysaccharide (OPS) isolated by mild acid hydrolysis of Azospirillum brasilense SR75 cell mass. After the OPS hydrolysis, D-rhamnose was recovered by gel-permeation chromatography on Toyopearl TSK HW-40 and was
Fitoterapia, 83(1), 153-160 (2011-11-01)
Persimmon proanthocyanidin was fractionated on Toyopearl TSK-HW-50-F to yield a fraction with strong inhibition on the catalytic activity and edema-inducing activity and lethality of Chinese cobra PLA(2). Thiolysis suggested that the terminal units included C, EGCG and myricetin, and epicatechin
Protein expression and purification, 82(1), 150-154 (2012-01-10)
We have cloned ansB (YPTB1411) gene from Yersinia pseudotuberculosis Q66CJ2 and constructed stable inducible expression system that overproduce L-asparaginase from Y. pseudotuberculosis (YpA) in Escherichiacoli BL21 (DE3) cells. For purification of YpA we used Q-Sepharose and DEAE-Toyopearl column chromatography. We
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