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TOYOPEARL® HW-40C Bulk Media

phase hydroxylated methacrylate, bottle of 500 mL, 75 μm particle size

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别名:
TOYOPEARL® HW-40 Size Exclusion Media
UNSPSC代码:
41115700

描述

HW-40C

产品线

TOYOPEARL®

形式

slurry

包装

bottle of 500 mL

参数

3 bar max. pressure

技术

HPLC: suitable

基质

hydroxylated methacrylic polymer

基质活性基团

hydroxylated methacrylate phase

粒径

75 μm

孔径

100-7000 Da MW range

工作pH值

2-13

分离技术

size exclusion (SEC)

一般描述

Toyopearl HW-40 resin is a hydroxylated methacrylic polymer for size exclusion chromatography. It is typically used for high throughput desalting and buffer exchange. It also can remove surfactants such as Triton X100 from protein solutions. It is often used for smaller biomolecules with exclusion limits up to 10,000 Da.

应用

Toyopearl media fractionate mixtures of proteins and other large molecular weight compounds over a wide size range. Applications include separating RNA from protein, resolving oligosaccharides by degree of polymerization, and isolating agglutinin while maintaining a high hemagglutination titer.

产品规格

Clean in place with 0.5 M NaOH or 0.1 M HCl.

外形

Shipped in 20% (v/v) ethanol.

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法律信息

Toyopearl is a registered trademark of Tosoh Corporation

象形图

Flame

警示用语:

Warning

危险声明

危险分类

Flam. Liq. 3

WGK

WGK 3

闪点(°F)

104.0 °F

闪点(°C)

40 °C

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

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Shu-fen Xu et al.
Fitoterapia, 83(1), 153-160 (2011-11-01)
Persimmon proanthocyanidin was fractionated on Toyopearl TSK-HW-50-F to yield a fraction with strong inhibition on the catalytic activity and edema-inducing activity and lethality of Chinese cobra PLA(2). Thiolysis suggested that the terminal units included C, EGCG and myricetin, and epicatechin
M V Pokrovskaya et al.
Protein expression and purification, 82(1), 150-154 (2012-01-10)
We have cloned ansB (YPTB1411) gene from Yersinia pseudotuberculosis Q66CJ2 and constructed stable inducible expression system that overproduce L-asparaginase from Y. pseudotuberculosis (YpA) in Escherichiacoli BL21 (DE3) cells. For purification of YpA we used Q-Sepharose and DEAE-Toyopearl column chromatography. We
Olga N Smol'kina et al.
Carbohydrate research, 347(1), 161-163 (2011-11-22)
A method is developed for the preparation of D-rhamnose from an O-polysaccharide (OPS) isolated by mild acid hydrolysis of Azospirillum brasilense SR75 cell mass. After the OPS hydrolysis, D-rhamnose was recovered by gel-permeation chromatography on Toyopearl TSK HW-40 and was

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