GC Analysis of Cocaine in Urine on a 100% Methyl Column after SPME using 100 μm PDMS Fiber

技术

gas chromatography (GC): suitable
solid phase microextraction (SPME): suitable

试验参数

sample/matrix: 0.5 mL urine (250 ng each analyte, 20 μL 2.5% NaF) in 1 mL vial
SPME fiber: polydimethylsiloxane, 100 μm (57300-U)
extraction: immersion, 30 min
desorption process: 3 min, 240 °C
column: 100% methyl polysiloxane, 30 m × 0.32 mm I.D., 0.25 μm (Supelco equivalent, Equity-1, 28055-U)
oven: 120 °C to 280 °C at 10 °C/min
inj. temp.: splitless (splitter opened after 1 min), 240 °C
detector: NPD, 280 °C
carrier gas: helium, 3 mL/min

适用性

application for SPME

应用

forensics and toxicology