HPLC Analysis of PAHs in Water on SUPELCOSIL^™ LC-PAH after SPME using 100 μm PDMS Fiber

技术

HPLC: suitable
solid phase microextraction (SPME): suitable

试验参数

sample/matrix: 5 μL PAH mix (Cat. No. 4-8743) in 5 mL water
SPME fiber: polydimethylsiloxane, 100 μm (57301)
extraction: immersion, 30 min (rapid stirring)
desorption process: static, 200 μL acetonitrile:water, 40:60, 2 min
column: SUPELCOSIL LC-PAH, 15 cm × 4.6 mm I.D., 5 μm particles (58318)
gradient: 0 min - 50% A; 5 min - 50% A; 30 min - 100% A; 45 min - 100% A
flow rate: 0-2 min: 0.2 mL/min 2-45 min: 1.0 mL/min
mobile phase: [A] acetonitrile:[B] water
detector: UV, 254 nm

适用性

application for HPLC
application for SPME

应用

environmental