HPLC Analysis of pBr322 DNA-Hae III Digest on TSKgel^® DEAE-NPR

技术

HPLC: suitable

试验参数

column: TSKgel DEAE-NPR, 3.5 cm × 4.6 mm I.D., 2.5 μm particles (with 5 mm DEAE-NPR guard column) (813075)
mobile phase: [A] 1 M sodium chloride/25 mm Tris-HCl, pH 9.0; [B] 25 mM Tris-HCl, pH 9.0
flow rate: 1 mL/min
column temp.: ambient
detector: UV, 260 nm
injection: 20 μL of 50 μg/mL Hae III digest of pBR322 DNA

适用性

application for HPLC

应用

life science and biopharma