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Merck
CN

66969-U

BIOshell™ A160 Peptide CN (2.7 μm) HPLC Columns

L × I.D. 15 cm × 2.1 mm, HPLC Column

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UNSPSC Code:
41115700
NACRES:
SB.52

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产品名称

BIOshell™ A160 Peptide CN, 2.7 μm HPLC Column, 2.7 μm particle size, L × I.D. 15 cm × 2.1 mm

material

stainless steel hardware (column)

agency

suitable for USP L10

description

Shell thickness (0.5 μm)
Solid Core (1.7 μm)

feature

endcapped

analyte chemical class(es)

peptides

packaging

1 ea of

extent of labeling

2.2% carbon loading

parameter

600 bar max. pressure
90 °C max. temp.

technique(s)

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

L × I.D.

15 cm × 2.1 mm

surface area

90 m2/g

surface coverage

2.2 μmol/m2

matrix

spherical silica particle platform
superficially porous particle

matrix active group

cyano phase

particle size

2.7 μm

pore size

160 Å

operating pH range

1-8

separation technique

hydrophilic interaction (HILIC)
normal phase
reversed phase

Quality Level

100

product line

BIOshell™

manufacturer/tradename

BIOshell™

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Other Notes

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Application

UHPLC Analysis of Peptides on BIOshell™ A160 Peptide C18, CN, and Phenyl-Hexyl, 2.7 μm

General description

BIOshell™ 160Å Peptide CN columns provide an alternative selectivity to the traditional C18 bonded phases. The introduction of the cyano group enables peptides and small proteins to be separated due to a combination of hydrophobicity and dipole-dipole interactions making this column an excellent choice for biomolecules that are not resolved on traditional C18 columns. These columns contain advanced Fused-Core particles with pores strategically sized to 160 Å to enable optimized peptide diffusion. This attribute makes these columns an excellent choice for peptide mapping. Additionally, the sterically-protected cyanopropyl ligands provide extra stability allowing the columns to be used at an extended pH range (2-9) and high temperatures (up to 90 °C).

Legal Information

BIOshell is a trademark of Sigma-Aldrich Co. LLC

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
USNWN001279
USNWN001278
USNWN001277
USNWN001276
USNWN001275

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商品

A Comparison of Superficially Porous Particle-based Column Chemistries for Peptide Mapping

This application note compares three different column chemistries from the BIOshell™ line of columns with superficially porous particles to evaluate their performance in peptide mapping.

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