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66905-U

Supelco

BIOshell A160 Peptide C18 (2.7 μm) HPLC Columns

L × I.D. 15 cm × 2.1 mm, HPLC Column

别名:

Superficially Porous Wide Pore C18 UHPLC Column

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About This Item

UNSPSC代码:
41115700
NACRES:
SB.52

产品名称

BIOshell A160 Peptide C18, 2.7 μm HPLC Column, 2.7 μm particle size, L × I.D. 15 cm × 2.1 mm

物料

stainless steel hardware

质量水平

100

Agency

suitable for USP L1

描述

Shell thickness (0.5 μm)
Solid Core (1.7 μm)

产品线

BIOshell
BIOshell

特点

endcapped: no

分析物化学类别

peptides
peptides

制造商/商品名称

BIOshell
BIOshell

包装

1 ea of

标记范围

4.6% carbon loading

参数

600 bar max. pressure
90 °C max. temp.

技术

HPLC: suitable
HPLC: suitable
LC/MS: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
UHPLC: suitable

长度 × 内径

15 cm × 2.1 mm

表面积

90 m2/g

表面覆盖度

2.2 μmol/m2

基质

spherical silica particle platform
superficially porous particle
superficially porous particle

基质活性基团

C18 (octadecyl) bonding phase, diisobutyloctadecyl

粒径

2.7 μm
2.7 μm

孔径

160 Å

工作pH范围

1-8

分离技术

reversed phase
reversed phase

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相关类别

一般描述

BIOshell 160 Å Peptide C18 columns are specifically engineered to provide efficient separation of peptides as well as small proteins. These columns contain advanced Fused-Core particles with pores strategically sized to 160 Å to enable optimized peptide diffusion. This attribute makes these columns an excellent choice for peptide mapping. Additionally, the sterically-protected C18 ligands provide extra stability allowing the columns to be used at an extended pH range (2-9) and high temperatures (up to 90 °C). This greatly expands the application range for the separation of biomolecules.

应用

UHPLC Analysis of Peptides on BIOshell A160 Peptide C18, CN, and Phenyl-Hexyl, 2.7 μm
BIOshell A160 Peptide C18, 2.7 μm HPLC Column, a non-end capped column that has superficially porous particle with C18 alkyl ligand as the stationary phase, is used mainly in the separation of peptides at a good flow rate.

特点和优势

BIOshell A160 Peptide C18, 2.7 μm HPLC Columns are compatible both with UHPLC and HPLC systems. They are specifically engineered for the fast and high-resolution seperation of biomolecules. These columns operate at high flow rates and have 40% higher efficiency than columns with fully porous particles of the same size. They are stable at high temperatures, which increases the throughput and enhances the peak shape.

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分析证书(COA)

Lot/Batch Number
USIIT001496
USIIT001495
USIIT001494
USIIT001492
USIIT001491

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Collagen-derived X-Hyp-Gly-type tripeptides promote differentiation of MC3T3-E1 pre-osteoblasts
Taga Y, et al.
Journal of functional foods, 46, 456-462 (2018)
Jennifer K Field et al.
Journal of chromatography. A, 1622, 461093-461093 (2020-04-29)
The Peptide RPC Column Characterisation Protocol was applied to 38 stationary phases, varying in ligand chemistry, base silica, end capping and pore size, which are suitable for the analysis of peptides. The protocol at low and intermediate pH is based

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A Comparison of Superficially Porous Particle-based Column Chemistries for Peptide Mapping

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Teriparatide Peptide Mapping Analysis by LC-UV-MS

LC-UV-MS workflow details teriparatide peptide mapping, including enzymatic digestion, separation conditions, and QTOF mass spectrometer identification.

Protein Fingerprinting of a Viral Vector, AAV5

Method development for protein fingerprinting of AAV serotype 5 using both intact mass analysis and peptide mapping, to determine critical quality attributes for gene therapy, utilizing three different columns.

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