产品名称
BIOshell™ A400 Protein C4, 3.4 μm HPLC Column, 3.4 μm particle size, L × I.D. 10 cm × 2.1 mm
material
stainless steel hardware
agency
suitable for USP L26
description
Shell thickness (0.2 μm)
Solid Core (3.0 μm)
feature
endcapped
packaging
1 ea of
extent of labeling
0.4% carbon loading
parameter
600 bar max. pressure
90 °C max. temp.
technique(s)
HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
L × I.D.
10 cm × 2.1 mm
surface area
15 m2/g
surface coverage
4.2 μmol/m2
matrix
spherical silica particle platform
superficially porous particle
matrix active group
C4 (butyl) phase
particle size
3.4 μm
pore size
400 Å
operating pH range
2-9
separation technique
reversed phase
Quality Level
product line
BIOshell™
manufacturer/tradename
BIOshell™
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General description
Legal Information
商品
BIOshell™ A400 Protein C4 columns contain 3.4 μm particles with 400 Å pores, which are derivatized with dimethylbutyl silane and exhaustively endcapped for optimum protein recovery.
The article describes the use of difluoroacetic acid (DFA) as an effective alternative to acid modifiers, formic acid (FA) and trifluoroacetic acid (TFA) in the LC-UV/MS analysis of proteins.
For use as a marker in SDS-PAGE; Albumin from chicken egg white, For use as a marker in SDS-PAGE; L-Lactic Dehydrogenase from rabbit muscle, Type XI, lyophilized powder, 600-1,200 units/mg protein
Optimization of a Reversed-Phase Liquid Chromatographic (RP-LC) method for the intact analysis of a therapeutic monoclonal antibody, trastuzumab, using a BIOshell™ A400 Protein C4 column.
实验方案
BIOshell™ IgG 1000 Å C4 UHPLC Column for Improved Biomacromolecule Separations
HPLC Analysis of the Monoclonal Antibody (mAb) Erbitux (Cetuximab) on BIOshell™ A400 Protein C4
Larger porous shell particles in BIOshell™ columns improve efficiency in U/HPLC analysis of peptides and proteins.
相关内容
High performance liquid chromatography (HPLC) can be used to separate and identify different large biomolecules such as protein and peptides in a sample. It is based on the pumping of a sample with a solvent (mobile phase) through a column packed with sorbent material (stationary phase) at a high pressure.
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