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58354

Supelco

SUPELCOSIL™ LC-8-DB (5 µm) HPLC Columns

L × I.D. 25 cm × 4.6 mm, HPLC Column

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About This Item

UNSPSC代码:
41115700
eCl@ss:
32110501

product name

SUPELCOSIL™ LC-8-DB HPLC 色谱柱, 5 μm particle size, L × I.D. 25 cm × 4.6 mm

Agency

suitable for USP L7

质量水平

100

特点

endcapped

制造商/商品名称

SUPELCOSIL™

标记范围

6.0% carbon loading

参数

0-70 °C temperature
400 bar pressure (5801 psi)

技术

HPLC: suitable

长度 × 内径

25 cm × 4.6 mm

表面积

170 m2/g

表面覆盖度

surface coverage 3.2 μmol/m2

基质

silica gel, spherical particle platform

基质活性基团

C8 (octyl) phase

粒径

5 μm

孔径

120 Å

应用

food and beverages

分离技术

reversed phase

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相关类别

一般描述

SUPELCOSIL LC-DB 针对碱性化合物的分析进行了特殊的去活化处理。与常规反相色谱柱相比，分析有机碱时该色谱柱保留时间更短、峰形更好和柱效更高。

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法律信息

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC

Choose from one of the most recent versions:

分析证书(COA)

Lot/Batch Number
291695-04
291695-03
291695-02
291695-01
291694-04

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If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What does DB stand for in Supelcosil LC-8DB HPLC column, Product 58354?

    DB stands for "deactivated base", and refers to the base treatment of the packing in this column. Without this treatment, basic compounds will adsorb to active acidic silanol sites.  If starting a new method, Supelco suggests using a new-technology/pure-silica C8, such as the Ascentis C8.

  4. Can the Supelcosil LC-8DB HPLC column, Product 58354, be used for acidic compounds?

    No, it is base treated, therefore acidic compounds will be adsorbed by the base treatment, resulting is peak tailing.  

  5. Do you have a cleaning procedure for Supelcosil LC-8DB HPLC column, Product 58354?

    Yes.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Vesna Cerkvenik-Flajs et al.
Analytica chimica acta, 663(2), 165-171 (2010-03-09)
An analytical method has been developed for the simultaneous determination of the following endectocide drugs in milk: ivermectin, abamectin, doramectin, moxidectin, eprinomectin, emamectin and nemadectin. Samples were extracted with acetonitrile, purified with solid-phase extraction on a reversed phase C(8), derivatised
J H Nichols et al.
Clinical chemistry, 40(7 Pt 1), 1312-1316 (1994-07-01)
This automated assay determines the concentration of the antidepressant fluoxetine (Prozac) and its active metabolite norfluoxetine in serum by reversed-phase HPLC with spectrophotometric detection. Extraction, injection, and quantification are performed by the Gilson Aspec automated sample handler. The patient's specimen
Jadwiga Piwowarska et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 805(1), 1-5 (2004-04-29)
A high-performance liquid chromatographic method is described for determination of lidocaine (2-(dietyloamino)-N-(2,6-dimetylofenylo) acetamid) and its metabolite, monoethylglycine xylidide (MEGX), in human serum containing various concentration of bilirubin. Lidocaine and its metabolite were extracted from human serum using dichloromethane. After separation
P K Kunicki
Journal of chromatography. B, Biomedical sciences and applications, 750(1), 41-49 (2001-02-24)
A HPLC-UV determination of clobazam and N-desmethylclobazam in human serum and urine is presented. After simple liquid-liquid extraction with dichloromethane the compounds and an internal standard diazepam were separated on a Supelcosil LC-8-DB column at ambient temperature under isocratic conditions
L Dal Bo et al.
Journal of chromatography. B, Biomedical applications, 665(2), 404-409 (1995-03-24)
A simple HPLC method has been developed for the determination of ticlopidine in human plasma. Plasma samples were buffered at pH 9 and extracted with n-heptane-isoamyl alcohol (98.5:1.5, v/v). Imipramine was used as internal standard. Chromatography was performed isocratically with
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