product name
Ascentis® C8 HPLC 色谱柱, 3 μm particle size, L × I.D. 10 cm × 2.1 mm
物料
stainless steel column
质量水平
Agency
suitable for USP L7
产品线
Ascentis®
特点
endcapped
制造商/商品名称
Ascentis®
包装
1 ea of
标记范围
15% Carbon loading
参数
≤70 °C temp. range
400 bar pressure (5801 psi)
技术
HPLC: suitable
LC/MS: suitable
长度 × 内径
10 cm × 2.1 mm
表面积
450 m2/g
表面覆盖度
4.0 μmol/m2
杂质
<5 ppm metals
基质
fully porous particle
silica gel high purity, spherical
基质活性基团
C8 (octyl) phase
粒径
3 μm
孔径
100 Å
operating pH range
2-8
应用
food and beverages
分离技术
reversed phase
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一般描述
Ascentis 系列色谱柱是 Supelco 科学家推出的第四代 HPLC 色谱柱技术。Ascentis 系列色谱柱是以高纯硅胶为基质，孔径 100Å，硅胶粒径为 3、5 和 10 微米。该色谱柱用于小分子的应用，柱内径可从微径柱（1.0mm 内径）扩展到制备尺寸（50mm 内径）。该系列包括 C18、C8、苯基、Si 和内嵌极性基团相（反相酰胺）。
Ascentis C8 色谱柱重现性好，对极性化合物具有优良的选择性。该键合相在高含水量的流动相和高有机相含量的流动相中均有优良的性能。
Ascentis C8 色谱柱重现性好，对极性化合物具有优良的选择性。该键合相在高含水量的流动相和高有机相含量的流动相中均有优良的性能。
特点和优势
- 对疏水性分子的保留性极好
- 峰形极佳
- LC-MS 分离流失低
法律信息
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Ebenezer B Asafu-Adjaye et al.
Journal of pharmaceutical and biomedical analysis, 43(5), 1854-1859 (2007-02-16)
Gastrointestinal stability of venlafaxine was evaluated in vitro in simulated gastric (SGF) and intestinal (SIF) fluids using a stability indicating HPLC method. The method was validated using a 5 microm Ascentis C18 column (150 mm x 4.6 mm) and mobile
Mahmoud Hasan et al.
Journal of pharmaceutical and biomedical analysis, 100, 341-347 (2014-09-10)
Clodronate belongs to the class of bisphosphonates which are used for the treatment of bone disorders. Due to its high polarity it has a low and highly variable oral bioavailability which results in low plasma concentrations and requires sensitive bioanalytical
Sreenivasa Rao Chitturi et al.
Journal of pharmaceutical and biomedical analysis, 55(1), 31-47 (2011-02-15)
This paper proposes a simple and selective RP-HPLC method for the determination of process impurities and degradation products (degradants) of atazanavir sulfate (ATV) drug substance. Chromatographic separation was achieved on Ascentis(®) Express C8, (150mm×4.6mm, 2.7μm) column thermostated at 30°C under
Ugandar Reddy Inugala et al.
Journal of chromatographic science, 51(5), 453-459 (2012-10-13)
This paper describes the development of a rapid, novel, stability-indicating gradient reversed-phase high-performance liquid chromatographic method and associated system suitability parameters for the analysis of naproxcinod in the presence of its related substances and degradents using a quality-by-design approach. All
Luana Magalhães et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1008, 98-107 (2015-12-08)
Nucleoside triphosphate diphosphohydrolase (NTPDase) is an enzyme belonging to the apyrase family that participates in the hydrolysis of the nucleosides di- and triphosphate to the corresponding nucleoside monophosphate. This enzyme underlies the virulence of parasites such as Leishmania. Recently, an
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Read our Biofiles article on Analysis of Isoprenoid Pathway Metabolites by LC-MS.
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