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577528-U

Supelco

BIOshell A160 Peptide Phenyl-Hexyl (2.7 μm) HPLC Columns

L × I.D. 15 cm × 2.1 mm, HPLC Column

别名:

BIOshell A160 Peptide Phenyl-Hexyl, 2.7 μm HPLC Column

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About This Item

UNSPSC代码:
41115700
NACRES:
SB.52

product name

BIOshell A160 Peptide Phenyl-Hexyl, 2.7 μm HPLC Column, column L × I.D. 15 cm × 2.1 mm

物料

stainless steel column

Agency

suitable for USP L11

产品线

BIOshell

特点

endcapped

包装

1 ea of

标记范围

4.7% Carbon loading

技术

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

色谱柱长度 × 内径

15 cm × 2.1 mm

表面积

90 m2/g

基质

Fused-Core® particle platform
superficially porous particle

基质活性基团

Phenyl-Hexyl (dimethylphenyl-hexylsilane) bonding phase

粒径

2.7 μm

孔径

160 Å pore size

operating pH range

2-9

分离技术

reversed phase

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相关类别

一般描述

BIOshell A160 Peptide Phenyl-Hexyl is a high-speed, high-performance liquid chromatography column based on a 160 Å Fused-Core® particle design. The particle provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency for high molecular weight solutes (up to 20 kDa) due to the shallow diffusion paths in the 0.5 μm thick porous shell and the small overall particle size of 2.7 μm. The end-capped, phenyl-hexyl bonded phase of the Peptide Phenyl-Hexyl provides a stable, reversed phase packing with a pore structure and pore size that is optimized for reversed-phase HPLC separations of peptides and polypeptides, using typical acidic mobile phases favored for proteomic applications.

分析说明

The target analytes include peptides and polypeptides < 20 kDa.

法律信息

Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

法规信息

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分析证书(COA)

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商品

This application note compares three different column chemistries from the BIOshell™ line of columns with superficially porous particles to evaluate their performance in peptide mapping.

相关内容

High performance liquid chromatography (HPLC) can be used to separate and identify different large biomolecules such as protein and peptides in a sample. It is based on the pumping of a sample with a solvent (mobile phase) through a column packed with sorbent material (stationary phase) at a high pressure.

生物分子是活细胞产生的大分子聚合物,作为活生物体的基本组成部分执行许多生命必需的生物过程。尽管现有很多分离和分析技术可供选择,最常用的还是高效液相色谱法(HPLC)。

HPLC separates and identifies large biomolecules like proteins and peptides by pumping the sample through a sorbent-packed column.

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