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一般描述
PlatePrep 96 孔真空萃取装置，包括透明的丙烯酸材料顶盖，这使得进行 96 孔板固相萃取时可以很方便地观察流速。聚丙烯底座具有极好的抗化学腐蚀能力，同时有一个可拆解的真空表/减压阀能控制所有孔的流速。
这个组合紧密的 96 孔真空萃取装置连结一个 SPE 96 孔提取板能同时处理 96 个样品。单阀控制、平行处理能力和均匀的流体动力学特性可进行更方便的方法开发，减少干扰，提高重现性。没使用过的孔可以盖上盖以后再用。
PlatePrep 96 孔真空萃取装置起动包（产品目录号 575650-U）可供不熟悉 96 孔固相萃取技术的分析人员使用。起动包含有 96 孔固相萃取所需的 PlatePrep 真空萃取装置和关键必需附件。
这个组合紧密的 96 孔真空萃取装置连结一个 SPE 96 孔提取板能同时处理 96 个样品。单阀控制、平行处理能力和均匀的流体动力学特性可进行更方便的方法开发，减少干扰，提高重现性。没使用过的孔可以盖上盖以后再用。
PlatePrep 96 孔真空萃取装置起动包（产品目录号 575650-U）可供不熟悉 96 孔固相萃取技术的分析人员使用。起动包含有 96 孔固相萃取所需的 PlatePrep 真空萃取装置和关键必需附件。
应用
- Quantitation of Trastuzumab and an Antibody to SARS-CoV-2 in Minutes Using Affinity Membranes in 96-Well Plates.: Demonstrating the efficiency of 96-well plates with vacuum manifolds in rapid immunological assays, this study highlights a method to quickly quantify antibodies, crucial for responsive disease management and treatment verification in clinical and research settings (Tan et al., 2022).
- High-Throughput Flow-Through Direct Immunoassays for Targeted Bacteria Detection.: This research utilizes 96-well plate formats to enhance the throughput and sensitivity of bacterial detection assays. The integration of vacuum manifolds streamlines sample processing, which is vital for environmental monitoring and clinical diagnostics (Chorti et al., 2021).
- Sample Preparation for High-Throughput Urine Proteomics Using 96-Well Polyvinylidene Fluoride (PVDF) Membranes.: Exploring the capabilities of 96-well plates in proteomics, this study outlines methods for preparing urine samples for mass spectrometry, aiming to standardize high-throughput proteomic analyses, which are essential for biomarker discovery and disease diagnostics (Ahmed et al., 2021).
- MStern Blotting-High Throughput Polyvinylidene Fluoride (PVDF) Membrane-Based Proteomic Sample Preparation for 96-Well Plates.: Addressing the challenges in proteomic sample preparation, this study presents a novel method that uses 96-well plates to enhance the efficiency and reproducibility of protein analyses, crucial for biochemical research and therapeutic development (Berger et al., 2015).
- Semiautomated Sample Preparation for Protein Stability and Formulation Screening via Buffer Exchange.: This article showcases the use of 96-well vacuum manifolds in streamlining the buffer exchange process in protein formulation studies, a critical step in pharmaceutical development to ensure the stability and efficacy of therapeutic proteins (Ying et al., 2016).
Affinity purification of Car9-tagged proteins on silica-derivatized spin columns and 96-well plates.
Meng Xu et al.
Protein expression and purification, 170, 105608-105608 (2020-02-18)
The Car9 affinity tag is a dodecameric silica-binding peptide that can be fused to the N- and C-termini of proteins of interest to enable their rapid and inexpensive purification on underivatized silica in a process that typically relies on l-lysine
David Kvaskoff et al.
Clinical chemistry, 62(4), 639-646 (2016-02-19)
The noncalcemic actions of vitamin D in multiple organs are now widely recognized. Vitamin D status has been linked with a wide variety of conditions, which has led to an increasing demand for vitamin D screening. In particular, there is
商品
This Sigma-Aldrich article discusses how the HybridSPE-Phospholipid Technology works and how the phospholipids are removed.
Novel sample prep technique coupled with the unique selectivity of the Ascentis Express F5 column enables a fast and simplified bioanalytical method for associated vitamin D metabolites.
Using HybridSPE-Phospholipid, Ascentis Express, and Other LC-MS Workflow Components
实验方案
The conversion of clinical methods to LC/MS/MS offers advantages; however, is accompanied by a few limitations, notably interference effects from the endogenous sample matrix.
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