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Merck
CN

569223-U

Supelco

Discovery® C18 (5 µm) HPLC Columns

L × I.D. 10 cm × 4.6 mm, HPLC Column

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别名:
Discovery RP18 HPLC Column
UNSPSC代码:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

Discovery® C18 高效液相色谱柱, 5 μm particle size, L × I.D. 10 cm × 4.6 mm

物料

stainless steel column

质量水平

Agency

suitable for USP L1

产品线

Discovery®

特点

endcapped

制造商/商品名称

Discovery®

包装

1 ea of

标记范围

12% Carbon loading

参数

≤70 °C temp. range
400 bar pressure (5801 psi)

技术

HPLC: suitable
LC/MS: suitable

长度 × 内径

10 cm × 4.6 mm

表面积

300 m2/g

表面覆盖度

3 μmol/m2

杂质

<10 ppm metals

基质

silica gel, high purity, spherical particle platform
fully porous particle

基质活性基团

C18 (octadecyl) phase

粒径

5 μm

孔径

180 Å

operating pH range

2-8

应用

food and beverages

分离技术

reversed phase

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相关类别

一般描述

Discovery® C18 可用于针对 C18 的任何方法。该色谱柱具有对称的峰形、优良的重现性和稳定性等特点,适用于所有常规和高要求的 C18 方法,是此类方法的理想选择。

应用


  • Development of an HPLC-based guanosine monophosphate kinase assay and application to Plasmodium vivax guanylate kinase.: Utilizes the Discovery® C18 HPLC Column for developing a novel enzymatic assay, aiding in malaria research and potential therapeutic target exploration (Pedro et al., 2019).

特点和优势

  • 优良的重现性
  • 分析酸性和碱性化合物均可获得良好的峰形
  • 稳定性好、低流失,适用于 LC-MS 分析
  • 能够分离多肽和小分子蛋白质
  • 与很多同类 C18 色谱柱相比,疏水性更小,因此分析速度更快

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法律信息

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

法规信息

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Yoshinori Masukawa et al.
Journal of lipid research, 49(7), 1466-1476 (2008-03-25)
Ceramides (CERs) in human stratum corneum (SC) play physicochemical roles in determining barrier and water-holding functions of the skin, and specific species might be closely related to the regulation of keratinization, together with other CER-related lipids. Structures of those diverse
Pei Yuan et al.
Chemistry (Weinheim an der Bergstrasse, Germany), 15(42), 11319-11325 (2009-09-18)
This study systematically investigates periodic mesoporous organosilicas (PMOs) with controlled helical and concentric circular (CC) pore architectures prepared through a basic-catalyzed sol-gel process by using an achiral cationic surfactant trimethyloctadecylammonium bromide (C(18)TAB) as a structure-directing agent, perfluorooctanoic acid (PFOA) as
R Nageswara Rao et al.
Biomedical chromatography : BMC, 23(11), 1145-1150 (2009-05-16)
A highly sensitive and selective on-line two-dimensional reversed-phase liquid chromatography/electrospray ionization-tandem mass spectrometry (2D-LC-ESI/MS/MS) method was developed and validated to determine rifaximin in rat serum by direct injection. The 2D-LC-ESI/MS/MS system consisted of a restricted access media column for trapping
Pilar Heredia-Middleton et al.
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, 149(4), 552-556 (2008-02-01)
Heat shock protein 70 (HSP70, 70 kDa) is the most commonly expressed protein in response to thermal stress. The extent of its expression is associated with differences in environmental temperatures. We investigated the heat shock response in red blood cells
S Bartesaghi et al.
Journal of pharmaceutical and biomedical analysis, 24(5-6), 1137-1141 (2001-03-15)
Total plasma homocysteine (tHcy) in children may be an useful biochemical marker for genetic risk of premature cardiovascular disease. We reported a rapid, isocratic HPLC method able to process very small amount of newborn plasma samples. A blood sample from

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