product name
Discovery® BIO 大孔 C18 高效液相色谱柱, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
物料
stainless steel column
质量水平
Agency
suitable for USP L1
产品线
Discovery®
特点
endcapped
制造商/商品名称
Discovery®
包装
1 ea of
标记范围
9.2% Carbon loading
参数
0-70 °C temperature
400 bar pressure (5801 psi)
技术
HPLC: suitable
LC/MS: suitable
长度 × 内径
25 cm × 4.6 mm
表面积
100 m2/g
表面覆盖度
3.6 μmol/m2
基质
silica particle platform
fully porous particle
基质活性基团
C18 (octadecyl) phase
粒径
5 μm
孔径
300 Å
工作pH值
2-8
分离技术
reversed phase
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相关类别
一般描述
RP-HPLC 上获得的多肽谱图可提供许多关于蛋白质结构、稳定性和纯度等有价值的信息。为达到预期效果，RP-HPLC 色谱柱必须能够分离样品中高百分比的多肽。多肽含量越高，获得的信息越有用。Discovery BIO 大孔 C18 柱可从酶解物中获得分离度很高的 RP-HPLC 多肽谱图。对 Discovery BIO 大孔系列产品所采用的硅胶和键合相化学性质的改进，增加了色谱柱的柱效并减少了拖尾，因而提高分离度。另一个优点是流动相中不需要 TFA 即可分析多肽，从而增强了 LC-MS 信号。
应用
- Kinetic performance comparison of superficially porous, fully porous and monolithic reversed-phase columns by gradient kinetic plots for the separation of protein biopharmaceuticals.: This study provides a detailed kinetic performance comparison of different types of reversed-phase HPLC columns, including the Discovery® BIO Wide Pore C18 HPLC Column. The research highlights its efficiency and resolution in separating complex protein biopharmaceuticals, making it a valuable tool for analytical chemists in biopharmaceutical analysis (Jaag et al., 2022).
- RP-LC x RP-LC analysis of a tryptic digest using a combination of totally porous and partially porous stationary phases.: This paper investigates the use of reverse-phase liquid chromatography (RP-LC) with both totally porous and partially porous stationary phases, including the BIO Wide Pore C18 HPLC Column, to analyze tryptic digests. The findings demonstrate its robustness and high resolution in proteomics applications, enabling precise peptide mapping and protein characterization (Mondello et al., 2010).
法律信息
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany
Hannah F Speer et al.
Journal of animal science, 98(9) (2020-08-28)
This study assessed the relative bioavailability of guanidinoacetic acid (GAA) in cattle. Seven ruminally cannulated Holstein steers (initial body weight of 280 kg) were used in an experiment with a 5 × 5 Latin square design; the two additional steers
相关内容
DiscoveryBIO Wide Pore C18 products offered by Sigma-Aldrich.
Chromatograms
application for HPLC我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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