product name
Discovery ® HS F5 HPLC 色谱柱, 3 μm particle size, L × I.D. 10 cm × 4.6 mm
物料
stainless steel column
质量水平
Agency
suitable for USP L43
产品线
Discovery®
特点
endcapped
制造商/商品名称
Discovery®
包装
1 ea of
标记范围
12% Carbon loading
参数
≤70 °C temp. range
400 bar pressure (5801 psi)
技术
HPLC: suitable
LC/MS: suitable
长度 × 内径
10 cm × 4.6 mm
表面积
300 m2/g
表面覆盖度
4 μmol/m2
杂质
<10 ppm metals
基质
silica gel, high purity, spherical particle platform
fully porous particle
基质活性基团
PFP (pentafluorophenyl) phase
粒径
3 μm
孔径
120 Å
operating pH range
2-8
应用
food and beverages
分离技术
reversed phase
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相关类别
一般描述
将 C18 柱方法转换至 Discovery® HS F5 柱的指导原则：
通常，碱性化合物在 HS F5 色谱柱上的保留时间比在 C18 柱上更长。在 C18 柱上流动相中的有机相含量提高 5% 至 10% 通常可获得与 HS F5 柱类似的保留值。使用其他化合物所得的结果差异则很大。然而 log Po/w 值小于 2.5 的化合物在 HS F5 柱上的保留时间通常要长于 C18 柱。保留时间的差异程度与化合物的 logP o/w 值密切相关。
通常，碱性化合物在 HS F5 色谱柱上的保留时间比在 C18 柱上更长。在 C18 柱上流动相中的有机相含量提高 5% 至 10% 通常可获得与 HS F5 柱类似的保留值。使用其他化合物所得的结果差异则很大。然而 log Po/w 值小于 2.5 的化合物在 HS F5 柱上的保留时间通常要长于 C18 柱。保留时间的差异程度与化合物的 logP o/w 值密切相关。
Discovery® HS F5 键合相可提供与 C18 色谱柱截然不同的反相分离。由于 F5 色谱柱上各种化合物通常会在与 C18 相同的时间范围内洗脱出来，使得大部分的 C18 方法可轻松转换到 F5 上。
特点和优势
- 独特的选择性
- 保留能力与 C18 柱类似（有时需要洗脱能力更强的流动相）
- 峰形极佳
- 稳定性好、低流失，适用于 LC-MS 分析
- 粒径范围从 3μm 到 10μm 可供选择
法律信息
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany
Jennie Wang et al.
Journal of pharmaceutical and biomedical analysis, 124, 267-273 (2016-03-13)
An HPLC method for the assay of a DNA topoisomerase inhibitor, LMP776 (NSC 725776), has been developed and validated. The stress testing of LMP776 was carried out in accordance with International Conference on Harmonization (ICH) guidelines Q1A (R2) under acidic
Umberto M Battisti et al.
Journal of chromatography. A, 1443, 152-161 (2016-03-30)
A "heart-cut" two-dimensional achiral-chiral liquid chromatography triple-quadrupole mass spectrometry method (LC-LC-MS/MS) was developed and coupled to in vivo cerebral microdialysis to evaluate the brain response to the chiral compound (±)-7-chloro-5-(3-furanyl)-3-methyl-3,4-dihydro-2H-1,2,4-benzothiadiazine-1,1-dioxide ((±)-1), a potent positive allosteric modulator (PAM) of AMPA receptor.
Jana Maláková et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 853(1-2), 265-274 (2007-04-10)
Galantamine, an alkaloid isolated from the bulbs and flowers of Caucasian snowdrop (Galanthus woronowii, Amaryllidaceae) and related species, is employed in human medicine for the treatment of various neuromuscular and neurodegenerative diseases. After the administration, the products of oxidative biotransformation
Federica Pellati et al.
Journal of pharmaceutical and biomedical analysis, 48(2), 254-263 (2007-12-14)
In this study a pentafluorophenylpropyl (PFPP) stationary phase was applied to the fast and reliable qualitative and quantitative analysis of ephedrine alkaloids in Ephedra plant material and derivatives. A Discovery HS F5 column (150mmx4.6mm i.d., 5microm) was used, with an
Chromatographic separation of arsenic species with pentafluorophenyl column and application to rice.
Koji Baba et al.
Journal of chromatography. A, 1354, 109-116 (2014-06-21)
Arsenic species, including arsenous acid, arsenic acid, methylarsonic acid, and dimethylarsinic acid, were determined using HPLC-ICPMS. The species were separated with a Discovery HS F5 column and a simple, volatile, and isocratic mobile phase of 0.1% (v/v) formic acid and
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