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文件

565322-U

Supelco

Ascentis® RP-Amide (3 µm) HPLC Columns

L × I.D. 15 cm × 4.6 mm, HPLC Column

别名:

RP-Amide Reversed-Phase Chromatography Column

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About This Item

UNSPSC代码:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

Ascentis® RP-Amide HPLC 色谱柱, 3 μm particle size, L × I.D. 15 cm × 4.6 mm

物料

stainless steel column

质量水平

100

Agency

suitable for USP L60

产品线

Ascentis®

特点

endcapped

制造商/商品名称

Ascentis®

包装

1 ea of

标记范围

19.5% Carbon loading

参数

≤70 °C temp. range
400 bar pressure (5801 psi)

技术

HPLC: suitable
LC/MS: suitable

长度 × 内径

15 cm × 4.6 mm

表面积

450 m2/g

表面覆盖度

2.7 μmol/m2

杂质

<5 ppm metals

基质

fully porous particle
silica gel high purity, spherical

基质活性基团

amide, alkyl phase

粒径

3 μm

孔径

100 Å

operating pH range

2-8

应用

food and beverages

分离技术

reversed phase

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相关类别

一般描述

Ascentis 系列色谱柱是 Supelco 科学家推出的第四代 HPLC 色谱柱技术。Ascentis 系列色谱柱是以高纯硅胶为基质，孔径 100Å，硅胶粒径为 3、5 和 10 微米。该色谱柱用于小分子的应用，柱内径可从微径柱（1.0mm 内径）扩展到制备尺寸（50mm 内径）。该系列包括 C18、C8、苯基、Si 和内嵌极性基团相（反相酰胺）。

Ascentis RP-Amide 反相酰胺柱是新一代超低流失、嵌入极性基团 (EPG) 的键合相，该键合相可提供与常规C18不同的选择性，并提高极性化合物 HPLC 和 LC-MS 分析的分离度。Ascentis RP-Amide 反相酰胺柱是嵌入极性基团 HPLC 键合相的首选。

应用


  • Preparation of mixed-mode stationary phase for separation of peptides and proteins in high-performance liquid chromatography: Highlights the application of Ascentis® RP-Amide HPLC column in the development of mixed-mode stationary phases, further demonstrating its versatility in handling diverse biochemical compounds and its pivotal role in advancing pharmaceutical and proteomic research (Alharthi et al., 2022).

  • On-line hyphenation of solid-phase extraction to chromatographic separation of sulfonamides with fused-core columns in sequential injection chromatography: Showcases the efficacy of Ascentis® RP-Amide HPLC columns in environmental analysis, specifically for the detection of contaminants in complex matrices. This study underscores the column′s robustness and high-resolution capabilities, making it invaluable in environmental laboratories (Batista et al., 2015).

  • Determination of zearalenone and its metabolites in endometrial cancer by coupled separation techniques: Demonstrates the critical role of Ascentis® RP-Amide HPLC columns in clinical research, particularly in the sensitive and accurate measurement of biomarkers in cancer diagnostics. The column′s superior retention for polar compounds proves essential for precise biomedical analyses (Gadzała-Kopciuch et al., 2011).

特点和优势

  • 对极性化合物具有良好的保留性能和峰形
  • 100% 水兼容
  • 超低流失，与 LC-MS 兼容
  • 独特的选择性

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法律信息

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

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分析证书(COA)

Lot/Batch Number
291281-06
291281-05
291281-04
291281-03
291281-02

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Jakub Fibigr et al.
Journal of pharmaceutical and biomedical analysis, 151, 291-300 (2018-02-08)
The presented work describes the development and validation of a rapid UHPLC-UV method using a fused core particle column with an RP-Amide stationary phase for the separation and quantitative analysis of caffeoylquinic and di-caffeoylquinic acids in green coffee extracts. Three
Rihana Parveen Shaik et al.
Journal of pharmaceutical analysis, 3(6), 489-499 (2013-12-01)
Liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was used for simultaneous quantification of tolterodine and its metabolite 5-hydroxy methyl tolterodine in rat plasma. Tolterodine-d6 and 5-hydroxy methyl tolterodine-d14 were used as internal standards (IS). Chromatographic separation was performed on Ascentis Express

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