55276-U
HybridSPE®-Phospholipid solid phase extraction (SPE) Cartridge
Cartridge, bed wt. 30 mg, volume 1 mL, pk of 200, polypropylene material (hardware), PE frit (20 μm)
别名:
HybridSPE (phospholipid and protein removal) SPE cartridge tube, 6 mL
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About This Item
UNSPSC代码:
41115712
NACRES:
NB.21
product name
HybridSPE ®-磷脂SPE小柱, Cartridge, bed wt. 30 mg, volume 1 mL, pk of 200, polypropylene material (hardware), PE frit (20 μm)
物料
PE frit (20 μm)
polypropylene hardware
质量水平
组成
bed wt., 30 mg
包装
pk of 200
技术
solid phase extraction (SPE): suitable
体积
1 mL
基质活性基团
zirconia-based phase
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一般描述
HybridSPE-Phospholipid磷脂去除技术是一种简单且通用的样本制备平台，设计用于在 LC-MS 或 LC-MS/MS 分析之前，从生物血浆和血清中总体去除内源性蛋白和磷脂干扰。首先通过加入和混合酸化乙腈使生物血浆或血清发生蛋白沉淀。然后通过离心除去沉淀蛋白，并将所得上清液上样至 HybridSPE-Phospholipid 96 孔板或固相萃取柱上，其作为化学过滤器，专门靶向去除内源性样品磷脂。截留磷脂的原理是HybridSPE-Phospholipid固定相键合的专有氧化锆离子和所有磷脂都具有的磷酸根发生高度选择性的路易斯酸碱相互作用。得到的洗脱液可立即用于 LC-MS 或 LC-MS-MS 分析。
HybridSPE-Phospholipid 96 孔板和Ultra固相萃取柱有“孔内”/“柱内”沉淀法可供选择。即先将生物血浆/血清加入孔内或柱内，接着加入酸化乙腈（沉淀剂）。短暂混合/涡旋后，抽真空。96孔板和 Ultra固相萃取柱含有一系列低孔隙率疏水滤膜/筛板，这些填充柱床的滤膜/筛板组件作为深层过滤器，有助于在提取过程中同时去除磷脂和沉淀蛋白。标准 HybridSPE-Phospholipid磷脂固相萃取小柱需要采用“离柱”沉淀法。
HybridSPE-Phospholipid 96 孔板和Ultra固相萃取柱有“孔内”/“柱内”沉淀法可供选择。即先将生物血浆/血清加入孔内或柱内，接着加入酸化乙腈（沉淀剂）。短暂混合/涡旋后，抽真空。96孔板和 Ultra固相萃取柱含有一系列低孔隙率疏水滤膜/筛板，这些填充柱床的滤膜/筛板组件作为深层过滤器，有助于在提取过程中同时去除磷脂和沉淀蛋白。标准 HybridSPE-Phospholipid磷脂固相萃取小柱需要采用“离柱”沉淀法。
应用
- Less is more: a methodological assessment of extraction techniques for per- and polyfluoroalkyl substances (PFAS) analysis in mammalian tissues.: This study evaluates various extraction techniques, including the use of HybridSPE-Phospholipid, for analyzing PFAS in mammalian tissues. The research highlights the importance of effective phospholipid removal to ensure accurate PFAS quantification (Mertens et al., 2023).
- Rapid analysis of 65 pharmaceuticals and 7 personal care products in plasma and whole-body tissue samples of fish using acidic extraction, zirconia-coated silica cleanup, and liquid chromatography-tandem mass spectrometry.: The study includes HybridSPE-Phospholipid as part of the cleanup process, demonstrating its efficiency in removing phospholipids to improve the detection of pharmaceuticals and personal care products in biological samples (Tanoue et al., 2020).
- Multi LC-MS/MS and LC-HRMS Methods for Determination of 24 Mycotoxins including Major Phase I and II Biomarker Metabolites in Biological Matrices from Pigs and Broiler Chickens.: This research utilizes HybridSPE-Phospholipid for the cleanup of biological matrices, enhancing the accuracy of mycotoxin detection by effectively removing interfering phospholipids (Lauwers et al., 2019).
- Effective phospholipid removal from plasma samples by solid phase extraction with the use of copper (II) modified silica gel cartridges.: The study compares various phospholipid removal techniques, highlighting the performance of HybridSPE-Phospholipid in achieving high phospholipid removal efficiency from plasma samples (Flieger et al., 2017).
- Liquid chromatography mass spectrometry determination of perfluoroalkyl acids in environmental solid extracts after phospholipid removal and on-line turbulent flow chromatography purification.: The research demonstrates the application of HybridSPE-Phospholipid in the removal of phospholipids from environmental solid extracts, facilitating the accurate measurement of perfluoroalkyl acids (Mazzoni et al., 2016).
特点和优势
- 融合了蛋白质沉淀的简单性和固相萃取(SPE)定向除去磷脂的选择性
- 通过完全除去磷脂和沉淀蛋白，减少离子抑制
- 2-3 步通用流程
- 方法开发最少，甚至不需要方法开发
- 有 96 孔和 1 mL 萃取柱规格可供选择
法律信息
HybridSPE is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
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Selective extraction and enrichment of nerve agent degradation products has been achieved using zirconia based commercial solid-phase extraction cartridges. Target analytes were O-alkyl alkylphosphonic acids and alkylphosphonic acids, the environmental markers of nerve agents such as sarin, soman and VX.
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商品
An article focusing on ion-suppression and phospholipid contamination and some of their major causes and difficulties.
实验方案
A simple method to enrich phospholipids from plasma samples, involving a HybridSPE-PPT 96-well plate that both retains phospholipids and removes precipitated proteins.
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