产品名称
HybridSPE®-Phospholipid Ultra磷脂固相萃取柱, cartridge, bed wt. 30 mg, volume 1 mL, pk of 100
Quality Level
material
PE frit (5-9 μm), polypropylene hardware
composition
bed wt., 30 mg
packaging
pk of 100
technique(s)
solid phase extraction (SPE): suitable
volume
1 mL
matrix active group
zirconia-based phase
General description
HybridSPE-Phospholipid磷脂去除技术是一种简单且通用的样本制备平台，设计用于在 LC-MS 或 LC-MS/MS 分析之前，从生物血浆和血清中总体去除内源性蛋白和磷脂干扰。首先通过加入和混合酸化乙腈使生物血浆或血清发生蛋白沉淀。然后通过离心除去沉淀蛋白，并将所得上清液上样至 HybridSPE-Phospholipid 96 孔板或固相萃取柱上，其作为化学过滤器，专门靶向去除内源性样品磷脂。截留磷脂的原理是HybridSPE-Phospholipid固定相键合的专有氧化锆离子和所有磷脂都具有的磷酸根发生高度选择性的路易斯酸碱相互作用。得到的洗脱液可立即用于 LC-MS 或 LC-MS-MS 分析。
HybridSPE-Phospholipid 96 孔板和Ultra固相萃取柱有“孔内”/“柱内”沉淀法可供选择。即先将生物血浆/血清加入孔内或柱内，接着加入酸化乙腈（沉淀剂）。短暂混合/涡旋后，抽真空。96孔板和 Ultra固相萃取柱含有一系列低孔隙率疏水滤膜/筛板，这些填充柱床的滤膜/筛板组件作为深层过滤器，有助于在提取过程中同时去除磷脂和沉淀蛋白。标准 HybridSPE-Phospholipid磷脂固相萃取小柱需要采用“离柱”沉淀法。
HybridSPE-Phospholipid 96 孔板和Ultra固相萃取柱有“孔内”/“柱内”沉淀法可供选择。即先将生物血浆/血清加入孔内或柱内，接着加入酸化乙腈（沉淀剂）。短暂混合/涡旋后，抽真空。96孔板和 Ultra固相萃取柱含有一系列低孔隙率疏水滤膜/筛板，这些填充柱床的滤膜/筛板组件作为深层过滤器，有助于在提取过程中同时去除磷脂和沉淀蛋白。标准 HybridSPE-Phospholipid磷脂固相萃取小柱需要采用“离柱”沉淀法。
Features and Benefits
- Ultra固相萃取柱可用于“柱内”蛋白沉淀法。
- 滤液极其洁净、无颗粒，适合直接进样用于UHPLC（超高效液相色谱）类分析。
- 融合了蛋白质沉淀的简单性和固相萃取(SPE)定向除去磷脂的选择性
- 通过完全除去磷脂和沉淀蛋白，减少离子抑制
- 2-3 步通用流程
- 方法开发最少，甚至不需要方法开发
- 有 96 孔和 1 mL 萃取柱规格可供选择
Legal Information
HybridSPE is a registered trademark of Merck KGaA, Darmstadt, Germany
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
实验方案
A simple method to enrich phospholipids from plasma samples, involving a HybridSPE-PPT 96-well plate that both retains phospholipids and removes precipitated proteins.
商品
This Sigma-Aldrich article continues to detail new methodology for the analysis of Vitamin D metabolites using HybridSPE-Phospholipid technology.
An article focusing on ion-suppression and phospholipid contamination and some of their major causes and difficulties.
Pankaj K Kanaujia et al.
Journal of chromatography. A, 1218(38), 6612-6620 (2011-08-25)
Selective extraction and enrichment of nerve agent degradation products has been achieved using zirconia based commercial solid-phase extraction cartridges. Target analytes were O-alkyl alkylphosphonic acids and alkylphosphonic acids, the environmental markers of nerve agents such as sarin, soman and VX.
A high-throughput method for liquid chromatography-tandem mass spectrometry determination of plasma alkylresorcinols, biomarkers of whole grain wheat and rye intake
Ross, A.B., et al.
Analytical Biochemistry, 499, 1-7 (2016)
Identification and elimination of ion suppression in the quantitative analysis of sirolimus in human blood by LC/ESI-MS/MS
Mano, N., et al.
Journal of Chromatography. B, Biomedical Applications, 879 (13-14), 968-974 (2011)
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| 55269-U | 04061832581996 |