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安全信息

54809

Sigma-Aldrich

安玛西亚离子交换色谱柱

10 μm particle size, L × I.D. 5 cm × 5 mm

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别名:
Amersham Biosciences Ion Exchange Columns
UNSPSC代码:
23151817
NACRES:
NA.56

长度 × 内径

5 cm × 5 mm

基质

MonoBeads

基质活性基团

amphoteric

粒径

10 μm

相容性

mode of use ion exchange

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一般描述

Mono 色谱柱是高效、pH 稳定的色谱柱,设计用于蛋白质、多肽和多核苷酸的高效离子交换分离。这些色谱柱的独特属性是基于单柱支撑体,即任何色谱支撑体中粒径分布最窄的珠状亲水性材料。这种单分散性允许在相对较低的反向压力下获得较高的流速。

应用

Mono columns are used in peptide mapping and monoclonal antibody purification. Mono P columns have been used to purify homogenous forms of fungal peroxygenase and to investigate separation of DNA restriction fragments.

法律信息

Mono P is a registered trademark of Cytiva

法规信息

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René Ullrich et al.
Biotechnology journal, 4(11), 1619-1626 (2009-07-09)
Extracellular peroxygenase from the agaric fungus Agrocybe aegerita is a versatile biocatalyst that oxygenates various substrates by means of hydrogen peroxide. The enzyme is routinely produced in suspensions of soybean meal and has until now been purified by several steps
E Westman et al.
Analytical biochemistry, 166(1), 158-171 (1987-10-01)
Separation of DNA restriction fragments by FPLC ion-exchange chromatography on Mono Q and Mono P columns was investigated. The columns were found to be particularly suitable for the separation of fragments up to 500-600 bp long. Larger fragments can also

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