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安全信息

54805

Sigma-Aldrich

Disposable PD-10 Desalting Column

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About This Item

UNSPSC代码:
13111000

物料

polypropylene (with polyethylene frit)

包装

pkg of 30 ea

储存温度

2-8°C

一般描述

The PD-10 desalting columns contain Sephadex® G-25 for gel filtration (size exclusion) of biomolecules. Globular compounds pass quickly through the column. Compounds, such as salts, pass through the column slowly. The column actually separates two classes of compounds by their molecular weight, thus desalting the protein of interest.

应用

Disposable PD-10 Desalting Column has been used:
  • to concentrate samples of high peroxidase activity from a PGF-HRP (prostaglandin F2α-horse radish peroxidase) conjugate solution and to remove free PGF from this solution.
  • for the separation of lanthanide-labeled proteins from unbound lanthanides by column chromatography.
  • for repurification of protein by removal of lower molecular weight fragments after elution from Ni-NTA agarose affinity column.
  • for separation of Eu-labeled protein from free Eu (Europium).
PD-10 desalting columns have been used to purify reduced Alemtuzumab (Campath, Berlex) humanized IgG1 rat monoclonal antibody from excess phospine, which is being developed for the treatment of chronic lymphocytic leukemia, autoimmune disease and for the prevention of transplant rejection.

法律信息

Sephadex is a registered trademark of Cytiva

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Warning

危险声明

危险分类

Aquatic Chronic 2 - Skin Sens. 1

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

新产品

历史批次信息供参考:

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Christophe M M Lahorte et al.
Journal of nuclear medicine : official publication, Society of Nuclear Medicine, 45(6), 1065-1073 (2004-06-08)
This study reports on the in vitro evaluation, biodistribution, and dosimetry of (123)I-labeled monoclonal antibody (mAb) 14C5, a new antibody-based agent proposed for radioimmunodetection of tumor growth and metastasis in vivo. (123)I-mAb 14C5 was prepared by direct iodination and tested
Large-Scale Production of Highly Active Recombinant Omi from Escherichia coli Expressed Soluble Protein Aggregates.
Rahman M, et al.
Biomedical Research, 23, 183-190 (2012)
Hemanta K Shrestha et al.
Biology of reproduction, 87(1), 18-18 (2012-05-04)
Horses are about five times more sensitive to the luteolytic effect of prostaglandin F2alpha (PGF) than cattle, as indicated by a recommended clinical dose of 5 mg in horses and 25 mg in cattle. Novel evaluations of the PGF plasma
Mario De Decker et al.
Nuclear medicine and biology, 35(5), 599-604 (2008-07-01)
Alemtuzumab (Campath, Berlex) is a humanized IgG1 rat monoclonal antibody directed against the cell surface CD52 antigen, found on lymphocytes and monocytes. It is being developed for the treatment of chronic lymphocytic leukemia (CLL), autoimmune disease and for the prevention
U Seitz et al.
European journal of nuclear medicine, 26(10), 1265-1273 (1999-12-14)
Anti-NCA antigen antibody BW 250/183 (Anti-Granulocyte) localizes more than 50% of injected antibody dose to the bone marrow. Therefore, this antibody is promising for adjuvant conditioning radioimmunotherapy of bone marrow before bone marrow transplantation. To examine its potential use for

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