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长度 × 内径
30 cm × 10 mm
基质活性基团
Superdex 200 HR10/300 GL phase
粒径
13-15 μm
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一般描述
Superdex prepacked columns are designed for high-performance, laboratory-scale separations of proteins, peptides, and other biomolecules according to size. High performance gel filtration offers fast separations with high resolution for a variety of applications including protein purification, studies of complex formation, and screening of uncharacterized samples.
Superdex 75 5/150 GL and Superdex 200 5/150 GL have small bed volumes, which is useful for a range of applications such as screening solubilization conditions for membrane proteins, the study of protein-protein interactions, and rapid purity check of proteins. These columns are an excellent choice when time and sample/buffer consumption is more important than the highest resolution since less buffer and sample volumes are required.
Superdex 75 5/150 GL and Superdex 200 5/150 GL have small bed volumes, which is useful for a range of applications such as screening solubilization conditions for membrane proteins, the study of protein-protein interactions, and rapid purity check of proteins. These columns are an excellent choice when time and sample/buffer consumption is more important than the highest resolution since less buffer and sample volumes are required.
与其他分子量范围为 100-7000 道尔顿的体积排阻色谱介质比较而言，Superdex Peptide 色谱柱可提供非常高的分离度，这使得它成为分离多肽类的最佳选择。Superdex 75 是分离低分子量重组蛋白质与多肽的单体和二聚体的理想工具。Superdex 200 适用于更大蛋白质的分析（例如，单克隆抗体）。
Superdex 75 HR10/300 GL 和 Superdex 200 HR10/300 GL 都是高分离度凝胶过滤色谱柱，适合微克至毫克级样品的高效分析和半制备应用。这些色谱柱还可用于常规 HPLC 和 FPLC™ 系统。HiLoad 是制备级 Superdex 75 和 Superdex 200 分离介质实验室规模的色谱柱。可被用于各种设备，包括单泵型配置和 FPLC、HPLC 与实验性规模的系统。
Superdex 75 HR10/300 GL 和 Superdex 200 HR10/300 GL 都是高分离度凝胶过滤色谱柱，适合微克至毫克级样品的高效分析和半制备应用。这些色谱柱还可用于常规 HPLC 和 FPLC™ 系统。HiLoad 是制备级 Superdex 75 和 Superdex 200 分离介质实验室规模的色谱柱。可被用于各种设备，包括单泵型配置和 FPLC、HPLC 与实验性规模的系统。
应用
Superdex prepacked columns are designed for high-performance, laboratory-scale separations of proteins, peptides, and other biomolecules according to size. Various applications include protein purification, studies of complex formation, and screening of uncharacterized samples.
特点和优势
- 在很广的分子量范围内都可以获得很高分离度（100 至 600,000 道尔顿）
- 分离快速
- 物理和化学稳定性好
- 色谱柱寿命长
- 色谱柱为生物兼容设计
- 易于扩大规模（HR 色谱柱至 HiLoad 色谱柱）
- 预装柱，使用方便重现性好
法律信息
FPLC is a trademark of Cytiva
Superdex is a registered trademark of Cytiva
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
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Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 879(27), 2935-2940 (2011-09-16)
Isolation of phosphatidyl-ethanolamine-binding protein-1 (PEBP-1) from bovine brain was described almost three decades ago but it required a large number of steps to reach high purity. After the fractionation of bovine testis proteins by ammonium sulfate precipitation we found that
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Journal of chromatography. A, 1218(47), 8503-8510 (2011-10-26)
Refolding enables bioprocesses predicated on proteins expressed as inclusion bodies in Escherichia coli. Optimization of size-exclusion chromatography (SEC) refolding is a significant challenge because a wide range of factors, including the choice of gel media, the column dimensions and configuration
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