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Merck
CN

54271-U

Supelco

Ascentis® Express 90 Å C18 (2.7 μm) HPLC Columns

L × I.D. 15 cm × 300 μm HPLC Capillary Column

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UNSPSC代码:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

Ascentis® Express C18 HPLC 毛细管柱, 2.7 μm particle size, L × I.D. 15 cm × 300 μm

物料

stainless steel column

质量水平

Agency

suitable for USP L1

产品线

Ascentis®

特点

endcapped

制造商/商品名称

Ascentis®

包装

1 ea of

参数

400 bar pressure (5801 psi)
60 °C temp. range

技术

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

长度 × 内径

15 cm × 300 μm

表面积

135 m2/g

杂质

<5 ppm metals

基质

Fused-Core particle platform
superficially porous particle

基质活性基团

C18 (octadecyl) phase

粒径

2.7 μm

孔径

90 Å pore size

工作pH值

2-9

应用

food and beverages

分离技术

reversed phase

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其他说明

The capillary housing for Ascentis® Express capillary columns is PEEK with stainless steel end fittings.

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法律信息

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

法规信息

新产品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is special about Ascentis Express?

    Ascentis Express columns provide a breakthrough in HPLC performance. Based on Fused-Core particle technology, Ascentis Express provides the benefits of sub-2 μm particles but at much lower backpressure.  These benefits include the capability of providing fast HPLC and higher resolution chromatography.  The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell.  A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.

  4. Can I use Ascentis Express on a UHPLC system?

    Yes.  Ascentis Express columns are packed in a way making them suitable for these ultra high pressure instruments.  In fact, Ascentis Express outperforms sub-2 μm micron columns on many applications since Ascentis Express provides the benefits of sub-2 μm particles but at much lower back pressure.  These benefits include the capability of providing fast HPLC and higher resolution chromatography.  The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell.  A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.

  5. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  6. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  7. What is the carbon load for Ascentis® Express C18 HPLC Columns?

    Carbon load which is commonly referred to as %Carbon is misleading and not meaningful for Ascentis® Express. Since Fused-Core particle is unique in the sense that it has a solid core and porous shell, the %Carbon number can not be compared to traditional porous particles.  The carbon coverage for Ascentis® Express C18 is 3.5 micromoles/meter-squared

  8. What is the pressure rating for 300 μm I.D. Ascentis® Express Capillary HPLC columns?

    The 300 μm I.D. Ascentis® Express Capillary HPLC columns are stable to operating pressures up to 6500 psi.

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Heena et al.
Journal of chromatographic science, 53(5), 800-806 (2014-10-18)
This study represents a new analytical high-performance liquid chromatography-fluorescence detector method for the determination of Al(III) as Al(III) complex with 8-hydroxyquinoline-5-sulfonic acid in a tap water sample and a coke sample. A micellar liquid chromatographic method is proposed for the
Petra Šilarová et al.
Food chemistry, 237, 471-480 (2017-08-03)
The degradation of catechins and other phenolics in green tea infusions were monitored using fast HPLC/MS separation. The final separation was performed within 2.5min using Ascentis Express C18 column (50mm×2.1mm i.d.) packed with 2μm porous shell particles. Degradation was studied
Pankaj Partani et al.
Journal of chromatographic science, 54(8), 1385-1396 (2016-05-27)
A liquid chromatography-tandem mass spectrometry method was developed and validated for the simultaneous determination of simvastatin (SV) and simvastatin acid (SVA) in human plasma. To improve assay sensitivity and achieve simultaneous analysis, SVA monitored in (-)ESI (electrospray ionization) mode within
Judy Stone
Methods in molecular biology (Clifton, N.J.), 1378, 301-320 (2015-11-26)
Serum from bar-coded tubes, and then internal standard, are pipetted to 96-well plates with an 8-channel automated liquid handler (ALH). The first precipitation reagent (methanol:ZnSO4) is added and mixed with the 8-channel ALH. A second protein precipitating agent, 1 %

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