product name
Ascentis® Express F5, 2.7 μm HPLC 色谱柱, 2.7 μm particle size, L × I.D. 7.5 cm × 3 mm
物料
stainless steel column
质量水平
Agency
suitable for USP L43
产品线
Ascentis®
特点
endcapped
制造商/商品名称
Ascentis®
包装
1 ea of
参数
60 °C temp. range
600 bar max. pressure (9000 psi)
技术
HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
长度 × 内径
7.5 cm × 3 mm
表面积
135 m2/g
杂质
<5 ppm metals
基质
Fused-Core particle platform
superficially porous particle
基质活性基团
PFP (pentafluorophenyl) phase
粒径
2.7 μm
孔径
90 Å pore size
工作pH值
2-8
应用
food and beverages
分离技术
reversed phase
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相关类别
一般描述
应用
- Liquid chromatographic determination of lumacaftor in the presence of ivacaftor and identification of five novel degradation products using high-performance liquid chromatography ion trap time-of-flight mass spectrometry.: This study utilizes the Ascentis® Express F5, 2.7 µm HPLC Column to develop a robust method for the determination of lumacaftor in the presence of ivacaftor, identifying five novel degradation products. The high resolution and efficiency of the column were crucial for the detailed analysis and identification process. (Özcan et al., 2023).
- Stability-indicating LC-MS/MS and LC-DAD methods for robust determination of tasimelteon and high resolution mass spectrometric identification of a novel degradation product.: The Ascentis® Express F5 column was employed to develop stability-indicating methods for tasimelteon, ensuring accurate determination and high-resolution identification of degradation products, highlighting the column′s effectiveness in stability studies and complex sample matrices. (Özcan et al., 2020).
- Recent trends in ultra-fast HPLC: new generation superficially porous silica columns.: This review discusses advancements in ultra-fast HPLC, focusing on new generation superficially porous silica columns like the Ascentis® Express F5. The article elaborates on the performance improvements and applications in analytical chemistry, emphasizing the column′s role in enhancing speed and resolution. (Ali et al., 2012).
法律信息
保护柱套
相关产品
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
Choose from one of the most recent versions:
分析证书(COA)
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Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
What is special about Ascentis Express?
Ascentis Express columns provide a breakthrough in HPLC performance. Based on Fused-Core particle technology, Ascentis Express provides the benefits of sub-2 μm particles but at much lower backpressure. These benefits include the capability of providing fast HPLC and higher resolution chromatography. The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell. A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
How can I measure my instrument bandwidth (IBW) and determine what columns can be used with minimal efficiency loss created by too much internal instrument volume?
The Guide to Dispersion Measurement has simple instructions on how to measure IBW and can be found at sigma-aldrich.com/express.
What is special about Ascentis® Express F5 HPLC Columns?
Ascentis® Express F5 HPLC Columns provide a breakthrough in HPLC performance. Based on Fused-Core particle technology, Ascentis® Express columns provide the benefits of sub-2 μm particles but at much lower backpressure. These benefits include the capability of providing fast HPLC and higher resolution chromatography. The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell. A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.
Are guard columns available for Ascentis® Express F5 3.0 mm ID HPLC Columns?
Yes, we have guard columns available for product Ascentis® Express F5 3.0 mm ID HPLC Columns. Product 53500-U is the Ascentis® Express Guard Holder and Product 53597-U Ascentis® Express F5 Guard Cartridge is a package of three guard cartridges, 2.7 μm particle size, L 5 mm × I.D. 3.0 mm .
Can I use Ascentis® Express F5 HPLC Columns on any type of HPLC system?
Ascentis® Express F5 HPLC Columns HPLC columns are capable of use on standard HPLC systems as well as UHPLC systems. These columns are packed in high pressure hardware capable of withstanding the pressures used in UHPLC systems.
Can I use Ascentis® Express F5 HPLC Columns on a UHPLC system?
Ascentis® Express F5 HPLC columns are packed in a way making them suitable for these ultra high pressure instruments. In fact, Ascentis® Express F5 HPLC Columns outperform sub-2 μm micron columns on many applications since Ascentis® Express F5 HPLC Columns provide the benefits of sub-2 μm particles but at much lower back pressure. These benefits include the capability of providing fast HPLC and higher resolution chromatography. The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell. A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.
What flow rate should I use with Ascentis® Express F5 HPLC columns?
Based on the minimum in the van Deemter curves, higher flows than 5um particle columns are required in order to maximize Ascentis® Express F5 HPLC column efficiency. The suggested starting point for flow rate for Ascentis® Express columns: 1.6 mL/min for 4.6 mm ID; 0.8 mL/min for 3.0 mm ID; and 0.4mL/min for 2.1 mm ID.
Do I need special fittings and tubing to connect Ascentis® Express F5 HPLC Columns?
While operating pressures may not exceed the 400 bar (6,000 psi) capability of your traditional instruments, sustained pressures of about 200 bar (3,000 psi) will exceed the recommended pressure for conventional PEEK tubing and fittings at the column inlet. We recommend changing to stainless steel fittings in all high pressure locations and have designed special High Performance HPLC Fittings/Interconnects that will stay tight at pressures of 1,000 bar (15,000 psi) or greater, even when elevated column temperatures are employed.
Is there anything special I need to do to my HPLC system to use Ascentis® Express F5 HPLC Columns?
Nothing special is required to use Ascentis® Express F5 HPLC Columns. To obtain the full benefits of Ascentis® Express Columns, one should minimize dispersion or instrument bandwidth in the HPLC system (tubing, detector flow cell) as well as confirm the detector response system is set at a fast level. For more information, request Guidelines for Optimizing Performance with Ascentis® Express HPLC Columns (T407102).
Can Ascentis® Express F5 HPLC columns be used for LC-MS?
Yes, Express Fused-Core™ particles were designed with LC-MS in mind. Even extremely short column lengths exhibit sufficient plate counts to show high resolving power. The flat van Deemter plots permit resolution to be maintained at very high flow rates to maximize sample throughput. All Ascentis stationary phases have been evaluated for MS compatibility during their development, and the Express phases are no exception. You can expect extremely low column bleed and background while maintaining longest possible column lifetime. A bonus of Ascentis® Express F5 HPLC Columns for high throughput UHPLC and LC-MS are that they are extremely rugged and highly resistant to plugging, a very common failure mode for competitor columns.
What is the pressure rating for Ascentis® Express F5 HPLC Columns?
Ascentis® Express F5 HPLC Columns are stable to operating pressures up to 600 bar (9000 psi).
What is the carbon load for Ascentis® Express F5 HPLC Columns?
Carbon load which is commonly referred to as %Carbon is misleading and not meaningful for Ascentis Express. Since Fused-Core™ particle is unique in the sense that it has a solid core and porous shell, the %Carbon number can not be compared to traditional porous particles. The carbon coverage for Ascentis® Express F5 HPLC columns is 3.5 micromoles/meter-squared.
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商品
Enhanced selectivity for stereoisomers and closely related compounds with alternate selectivity from C18 phases for basic, acidic, or neutral compounds
实验方案
Fast and Accurate LC-MS Analysis of Vitamin D Metabolites, Using Ascentis® Express F5 HPLC Columns from Supelco®
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