产品名称
Discovery® C18 高效液相色谱柱, 5 μm particle size, L × I.D. 25 cm × 4 mm
material
stainless steel column
agency
suitable for USP L1
product line
Discovery®
feature
endcapped
manufacturer/tradename
Discovery®
packaging
1 ea of
extent of labeling
12% Carbon loading
parameter
≤70 °C temp. range
400 bar pressure (5801 psi)
technique(s)
HPLC: suitable
LC/MS: suitable
L × I.D.
25 cm × 4 mm
surface area
300 m2/g
surface coverage
3 μmol/m2
impurities
<10 ppm metals
matrix
silica gel, high purity, spherical particle platform
fully porous particle
matrix active group
C18 (octadecyl) phase
particle size
5 μm
pore size
180 Å
operating pH range
2-8
application(s)
food and beverages
separation technique
reversed phase
Quality Level
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Application
- 多替拉韦强制降解产物的鉴定、分离和表征及其细胞毒性潜力。：采用Discovery® C18 HPLC色谱柱研究抗逆转录病毒药物多替拉韦的稳定性和降解，这对于确保药物安全性和有效性至关重要（Saida et al., 2019）。
- 重组人β淀粉样蛋白1-42的快速、精细和稳健的表达、纯化和表征方法。：该方法学研究利用Discovery® C18 HPLC色谱柱纯化和分析β样淀粉蛋白，为阿尔茨海默病的研究做出了贡献（Prakash et al., 2019）。
- 可作为药物研发工具的COMT活性HPLC电化学检测方法的开发和验证。展示了使用Discovery® C18 HPLC色谱柱开发的检测儿茶酚-O-甲基转移酶（COMT）活性的新型分析方法，该方法有助于精神和神经药物的开发（Viljoen et al., 2019）。
Features and Benefits
- 优良的重现性
- 分析酸性和碱性化合物均可获得良好的峰形
- 稳定性好、低流失，适用于 LC-MS 分析
- 能够分离多肽和小分子蛋白质
- 与很多同类 C18 色谱柱相比，疏水性更小，因此分析速度更快
General description
Discovery® C18 可用于针对 C18 的任何方法。该色谱柱具有对称的峰形、优良的重现性和稳定性等特点，适用于所有常规和高要求的 C18 方法，是此类方法的理想选择。
Legal Information
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany
wgk
WGK 1
存储类别
13 - Non Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
T Nagano et al.
Journal of dental research, 88(9), 823-828 (2009-09-22)
Mmp-20 and Klk4 are the two key enamel proteases. Can both enzymes process amelogenin to generate the major cleavage products that accumulate during the secretory stage of amelogenesis? We isolated Mmp-20 and Klk4 from developing pig teeth and used them
T R Shantha Kumar et al.
Journal of pharmaceutical and biomedical analysis, 38(1), 173-179 (2005-05-24)
A simple, precise and accurate isocratic reverse-phase liquid chromatography method with programmed wavelength detection has been validated to quantify DRF-4367 and Phenol red, simultaneously for application in rat in situ single pass intestinal perfusion study to assess intestinal permeability of
Yuan-Qing Xia et al.
Rapid communications in mass spectrometry : RCM, 20(12), 1831-1837 (2006-05-18)
A major challenge in selecting an appropriate stationary phase for diastereomeric separation is that it is difficult to predict which of the commercially available stationary phases could achieve the required liquid chromatographic (LC) separation. This work describes the selection and
Satoru Sonoke et al.
Cancer research, 68(21), 8843-8851 (2008-11-01)
The pharmacokinetics and antitumor activity of pegylated small interfering RNA (siRNA)/cationic liposome complexes were studied after systemic administration to mice. We designed pegylated-lipid carriers for achieving increased plasma concentrations of RNA and hence improved accumulation of RNA in tumors by
Pilar Heredia-Middleton et al.
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, 149(4), 552-556 (2008-02-01)
Heat shock protein 70 (HSP70, 70 kDa) is the most commonly expressed protein in response to thermal stress. The extent of its expression is associated with differences in environmental temperatures. We investigated the heat shock response in red blood cells
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