material
fused silica
agency
meets requirements for USP G3
parameter
0-250 °C temperature (isothermal or programmed)
Beta value
313
df
0.20 μm
technique(s)
gas chromatography (GC): suitable
L × I.D.
30 m × 0.25 mm
matrix active group
Non-bonded; poly(50% phenyl/50% methyl siloxane) phase
column type
capillary intermediate polar
General description
Application: The SP-2250 column is a non-bonded 50% phenyl polymer. It is highly effective for the analysis of polar compounds.
USP Code: This column meets USP G3 requirements.
Phase:
USP Code: This column meets USP G3 requirements.
Phase:
- Non-bonded
- Poly(50% phenyl/50% methyl siloxane)
- 0 °C to 250 °C (isothermal or programmed)
Application
Capillary column coated with SP®-2250 (methyl silicone-phenyl silicone, 1:1) stationary phase may be used in gas chromatographic analysis of azaarenes (benzacridines and dibenzacridines).
Other Notes
We offer a variety of chromatography accessories including analytical syringes
Legal Information
SP is a registered trademark of Sigma-Aldrich Co. LLC
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O W Lau et al.
Methods and findings in experimental and clinical pharmacology, 13(7), 475-481 (1991-09-01)
A gas-liquid chromatographic method was developed for the simultaneous assay of six local anesthetics, including amethocaine, bupivacaine, etidocaine, lignocaine, mepivacaine and prilocaine, in biological samples. These drugs and internal standard (clomipramine) in basified samples were extracted into 5 ml n-hexane
W Löscher
Therapeutic drug monitoring, 4(3), 315-318 (1982-01-01)
A rapid method was developed for the determination of diazepam, desmethyldiazepam, oxazepam, and 3-hydroxydiazepam (temazepam) in human plasma using electron capture gas chromatography. Following a single extraction with benzene from 0.2 ml plasma, diazepam and its metabolites were quantitated on
D F Tomkins
Journal - Association of Official Analytical Chemists, 70(6), 1056-1058 (1987-11-01)
An isothermal gas chromatographic method for measuring alachlor in Micro-Tech (microencapsulated) formulations was tested by 5 collaborators. The samples were prepared in acetone, and alachlor was determined using a gas chromatographic column of 10% SP-2250 on 100-120 mesh Supelcoport. Di-n-pentylphthalate
J E Wallace et al.
Clinical chemistry, 25(7), 1296-1300 (1979-07-01)
We describe the use of electron-capture gas chromatography or reversed-phase "high-performance" liquid chromatography for concurrent analysis for diazepam and nordiazepam in serum. In the gas-chromatographic analysis our use of a new chemically deactivated stationary liquid phase, SP 2250-DB (Supelco, Inc.)
V Gambaro et al.
Journal of analytical toxicology, 6(6), 321-323 (1982-11-01)
The gas chromatographic identification of barbiturates isolated from biological material is not optimal, and due to their polar nature, it is difficult to obtain good resolution on most columns. To improve resolution, flash alkylation appeared to be a reasonable alternative.
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