Anti-CXCL1/GROα Antibody, clone 2J19 ZooMAb^® Rabbit Monoclonal

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Clone 2J19 is a ZooMAb^® rabbit recombinant monoclonal antibody that detects CXCL1/GRO . It targets an epitope within 16 amino acids from the C-terminal region.

KLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal of human CXCL1/GRO .

Quality Control Testing

Evaluated by Western Blotting in BT-549 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CXCL1/GRO in BT-549 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected CXCL1/GRO in lysates from PC-3 cells and human spleen tissue.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected His-tagged, full-length, recombinant human CXCL1/GRO .

Affinity Binding Assay: A representative lot of this antibody bound with CXCL1/GRO peptide with at least ten thousand-fold (10,000X) higher than with non-specific control peptide.

Immunocytochemistry Analysis: A 1:100 Dilution from a representative lot detected CXCL1/GRO in PC-3 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CXCL1/GRO in human spleen and human colon cancer tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Growth-regulated alpha protein (UniProt: P09341; also known as C-X-C motif chemokine 1, CXCL1, GRO-alpha (1-73), Melanoma growth stimulatory activity, MGSA, Neutrophil-activating protein 3, NAP-3) is encoded by the CXCL1 (also known as GRO, GRO1, GROA, MGSA, SCYB1) gene (Gene ID: 2919) in human. CXCL1/GRO is a multi-functional chemokine that displays chemotactic activity for neutrophils. It is synthesized with a signal peptide (aa 1-34), which is subsequently cleaved off to produce the mature protein that can be further cleaved into three chains (aa 4-73; 5-73; and 6-73) via proteolytic cleavage after secretion from peripheral blood monocytes. CXCL1/GRO may play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. Its levels are reported to be low under basal conditions but increase significantly following active infection. It plays a role in recruiting and activating neutrophils for microbial killing at the tissue site and can also activate the release of proteases and reactive oxygen species. CXCL1/GRO signals through binding to CXCR2, a G-protein coupled receptor. Aberrant expression of CXCL1/GRO has been reported in several cancers and has been associated with oncogenesis, metastasis, angiogenesis and chemoresistance. Clone HL2401 is shown to specifically binds to CXCL1, although it may show slight binding to CXCL2. It is shown to reduce both cellular proliferation and invasion of cancer cells overexpressing CXCL1/GRO . It also induces apoptosis and impairs tumor vasculature. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Miyake, M., et al. (2019). Theranostics.;9(3):853-867; Yang C., et al. (2019). Int. J. Oncol. 55(3); 684-696Wuyts, A., et al. (1999). Eur. J. Biochem. 260(2); 421-429).

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.