Anti-ST2/IL-33R Antibody, clone 1N22 ZooMAb^® Rabbit Monoclonal

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Interleukin-1 receptor-like 1 (UniProt: Q01638; also known as Protein ST2) is encoded by the IL1RL1 (also known as DER4, ST2, T1) gene (Gene ID: 9173) in human. Interleukin-33 receptor (IL-33R) is a member of the IL-1 receptor family and plays a pivotal role in immune regulation. IL-33R forms a signaling complex by binding IL-33 and recruiting the IL-1 receptor accessory protein (IL-1RAcP), which facilitates downstream signal transduction. This interaction engages MyD88, leading to activation of NF- B and MAPK pathways, crucial for inflammatory responses. The receptor′s expression is predominantly observed on Th2 cells, regulatory T cells (Tregs), group 2 innate lymphoid cells (ILC2s), mast cells, and other leukocytes, underscoring its role in type 2 immunity. IL-33R mediates the effects of IL-33, a cytokine involved in promoting Th2-associated cytokine production, such as IL-4, IL-5, and IL-13. This axis is essential for the development of allergic inflammation, asthma, and other chronic inflammatory diseases. Additionally, IL-33R signaling contributes to tissue repair, parasite defense, and neuroimmune regulation. Dysregulation of IL-33R signaling has been implicated in various pathologies, including autoimmune diseases, cardiovascular diseases, and cancer. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Jiménez-Munguía, I., et al., (2022), Curr. Opin. Struct. Biol., 77, 102467).

KLH-conjugated linear peptide corresponding to 25 amino acids from the N-terminal extracellular domain of human ST2/IL-33R.

Quality Control Testing

Evaluated by Western Blotting using a His-tagged, recombinant fragment of human ST2/IL-33R protein.

Western Blotting Analysis: 1:10,000 of this antibody detected a His-tagged, recombinant fragment of human ST2/IL-33R protein.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected ST2/IL-33R in human kidney tissue lysate.

Affinity Binding Assay: A representative lot of this antibody bound with ST2/IL-33R peptide with at least one billion-fold (1,000,000,000X) higher affinity than with non-specific control peptide.

Enzyme Immunoassay: A variety dilutions of representative lot of this antibody detected ST2/IL-33R protein in ELISA application.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 1N22 is a ZooMAb^® rabbit recombinant monoclonal antibody that detects ST2/IL-33R. It targets an epitope within 25 amino acids from the N-terminal extracellular domain.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.