Anti-Properdin/CFP Antibody, clone 1K9 ZooMAb^® Rabbit Monoclonal

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Clone 1K9 is a ZooMAb^® recombinant rabbit monoclonal antibody that specifically detects Properdin. It targets an epitope within 16 amino acids from the N-terminal half.

KLH-conjugated linear peptide corresponding to 16 amino acids from the N-terminal half of human Properdin.

Quality Control Testing

Evaluated by Western Blotting in rat serum.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Properdin protein in rat serum.

Tested Applications

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Properdin/CFP in human peripheral blood mononuclear cells (PBMC).

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Properdin/CFP in human tonsil tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound Properdin/CFP peptide with at least ten thousand-fold (10,000X) higher than with non-specific control peptide.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Properdin (UniProt P27918; also known as Complement factor P) is encoded by the CFP (also known as PFC, PFD) gene (Gene ID 5199) in human. Properdin is a plasma glycoprotein and the only known positive regulator of the complement cascade. It binds and stabilizes the alternative pathway (AP) C3 convertase C3bBb and may also serve as a platform to form new C3bBb convertases on cell surface. Properdin-mediated AP activation plays an important role in host defense, and properdin deficiency (PFD) increases the risk for Neisseria meningitidis infection in human. Properdin is primarily expressed by leukocytes, including monocytes and T lymphocytes, and is secreted into the bloodstream. Subcellularly, it is localized in the extracellular space where it interacts directly with complement components on pathogen surfaces. Human properdin is produced with a signal peptide sequence (a.a. 1-27), which is removed posttranslationally to yield the mature protein (a.a. 28-469) composed entirely of seven TSP type-1 domains (domain 0 through 6; a.a. 28-462). TSP type-1 domains 0 and 1 binds to other TSP type-1 domains 4 and 6 respectively and mediate multimerization. Properdin-deficiency exacerbated disease condition in a murine model of factor H-(fH-) related C3 glomerulopathy, but prevented the development of allergen-induced airway inflammation. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Sun, Z., et al. (2004). J Mol Biol.343(5):1327-43).

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.