Anti-VASP Antibody, clone 1C6 ZooMAb^® Rabbit Monoclonal

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Clone 1C6 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Vasodilator-stimulated phosphoprotein (VASP). It targets an epitope within 24 amino acids from the C-terminal half.

KLH-conjugated linear peptide corresponding to 24 amino acids from the C-terminal half of human Vasodilator-stimulated phosphoprotein (VASP).

Quality Control Testing

Evaluated by Western Blotting in THP-1 cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected VASP in THP-1 cell lysate.

Tested Applications

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected VASP in A431 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected VASP in Human tonsil tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound VASP peptide with a KD of 2.6 x 10-7 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Vasodilator-stimulated phosphoprotein (UniProt: P50552; also known as VASP) is e encoded by the VASP gene (Gene ID: 7408) in human. VASP is an actin- and profilin-binding, homotetrameric protein that is expressed in platelets at high levels and plays a major role in negatively regulating secretory and adhesive events in these cells. It is involved in a wide range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation, and cell migration. It is reported to promote actin filament elongation and protect the barbed end of growing actin filaments against capping. It also increases the rate of actin polymerization in the presence of capping protein. It is shown to stimulate actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. It plays a critical role in actin dynamics in platelets and regulates platelet aggregation. It is a major substrate for PKA and PKG and is phosphorylated on Serine 157 by PKA and on serine 239 by PKG. In ADP-activated platelets, phosphorylation leads to fibrinogen receptor inhibition. Its phosphorylation on threonine 278 requires prior phosphorylation on serine 157 and 239. These phosphorylation events modulate F-actin binding, actin filament elongation, and platelet activation. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Wentworth, JKT., et al. (2006). Biochem. J. 393(2): 555-564; Chitaley, K., et al. (2004). FEBS Lett. 556(1); 211-215; Butt, E., et al. (1994). J. Biol. Chem. 269(20); 14509-14517).

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.