Anti-TBX21 Antibody, clone 1B21 ZooMAb^® Rabbit Monoclonal

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ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Clone 1B21 is a ZooMAb^® recombinant rabbit monoclonal antibody that specifically detects T-box protein 21 (TBX21) protein. It targets an epitope within 23 amino acids from the C-terminal half.

KLH-conjugated linear peptide corresponding to 23 amino acids from the C-terminal half of human T-box protein 21 (TBX21).

Quality Control Testing

Evaluated by Western Blotting in NK-92 cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected TBX21 in NK-92 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected TBX21 recombinant protein.

Flow Cytometry Analysis: 1 µg from a representative lot detected TBX21 in one million NK-92 cells.

Affinity Binding Assay: The binding affinity of this antibody to TBX21 peptide was at least ten thousand-fold (10,000X) higher than that obtained with the non-specific control peptide.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected TBX21 in HDLM-2 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

T-box transcription factor TBX21 ( UniProt: Q9UL17; also known as T-box protein 21) is encoded by TBX21 gene (Gene ID: 30009) in human. Its expression is primarily T-cell specific and also expressed in Bone marrow, lung and lymphoid tissue. It is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. Tbox genes encode transcription factors involved in the regulation of developmental processes. Studies in mouse show that TBX21 protein is a Th1 cell-specific transcription factor that controls the expression of the hallmark Th1 cytokine, interferon-gamma. Expression of the human ortholog also correlates with IFN-G expression in Th1 and natural killer cells, suggesting a role for this gene in initiating Th1 lineage development from naive Th precursor cells. TBX21 initiates TH1 lineage development from naive TH precursor cells both by activating TH1 genetic programs and by repressing the opposing TH2 programs. Its function is vital in the immune system, influencing the body′s ability to defend against pathogens and maintain immune homeostasis. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.