Anti-CYP46A1 Antibody, clone 1C20 ZooMAb^® Rabbit Monoclonal

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Clone is a ZooMAb^® rabbit recombinant monoclonal antibody that detects CYP46A1. It targets an epitope within 21 amino acids from the internal region of the protein.

KLH-conjugated linear peptide corresponding to 21 amino acids from the Internal region of human CYP46A1.

Quality Control Testing

Evaluated by Western Blotting in U-251 MG cell lysate.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected CYP46A1 in U-251 MG cell lysate.

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected CYP46A1 in lysate from human brain tissue and mouse brain tissue.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CYP46A1 in human cerebral cortex tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CYP46A1 in U-251 MG cells.

Affinity Binding Assay: A representative lot of this antibody bound CYP46A1 peptide with at least ten thousand-fold (10,000X) higher than with non-specific control peptide.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Cholesterol 24-hydroxylase (UniProt: Q9Y6A2; EC:1.14.14.25; also known as CH24H, Cholesterol 24-monooxygenase, Cholesterol 24S-hydroxylase, Cytochrome P450 46A1) is encoded by the CYP46A1 (also known as CYP46) gene (Gene ID: 10858) in human. CYP46A1 is a member of the cytochrome P450 superfamily of enzymes. These enzymes are characterized by their heme-binding domain, which is crucial for their enzymatic activity involving electron transfer. CYP46A1 is a membrane-associated protein predominantly located in the endoplasmic reticulum. Its structure facilitates the oxidation of cholesterol, a critical step in cholesterol homeostasis and metabolism. The primary function of CYP46A1 is to catalyze the conversion of cholesterol to 24S-hydroxycholesterol. This conversion is a key mechanism for the elimination of cholesterol from the brain, as 24S-hydroxycholesterol can cross the blood-brain barrier, unlike cholesterol itself. This enzymatic activity is vital for maintaining cholesterol balance within the central nervous system and influences various cellular processes including membrane fluidity and the formation of lipid rafts. Alterations in CYP46A1 activity and expression have been implicated in several neurodegenerative diseases. Reduced activity of CYP46A1 has been associated with Alzheimer s disease, potentially due to impaired cholesterol turnover in the brain, which affects the generation and clearance of amyloid-beta plaques.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.