Anti-Neurexin-1-β Antibody, clone 1F14 ZooMAb^® Rabbit Monoclonal

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ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Clone 1F14 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Neurexin-1β. It targets an epitope within 20 amino acids from the extracellular domain.

KLH-conjugated linear peptide corresponding to 20 amino acids from the extracellular domain of human Neurexin-1β.

Quality Control Testing

Evaluated by Western Blotting in Mouse brain tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Neurexin-1-β in Mouse brain tissue lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Neurexin-1-β in Human brain and Rat brain tissue lysates.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Neurexin-1-β in HeLa, HepG2, NIH 3T3, and U-87 MG cells.

Affinity Binding Assay: A representative lot of this antibody bound in Neurexin-1-β peptide with a KD of 7.6 x 10-8 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Neurexin-1-β in Human cerebral cortex.

Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Neurexin-1-β in Human cerebral cortex tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Neurexin-1-β (UniProt: P58400; also known as Neurexin I-beta) is encoded by the NRXN1 gene (Gene ID: 9378) in human. Neurexins are a large family of proteins that act as neuronal cell-surface receptors. Vertebrates contain three large neurexin genes, each of which expresses longer - and shorter β-neurexins, producing six principal neurexins (neurexins 1 , 2 , 3 and 1β , 2β, 3β). β-neurexins serve as receptors for neuroligin-1, post-synaptic membrane protein that triggers synapse formation at axon contacts. Within the presynaptic terminal, it is reported to be involved in the recruitment of scaffolding proteins and synaptic vesicles. Neurexin-1-β is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-50), which is subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 51-396), a transmembrane domain (aa 397-417), and a cytoplasmic domain (aa 418-472). Six isoforms of Neurexin-1-β have been described that are produced by alternative promoter usage and alternative splicing. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Reissner, C., et al. (2013). Genome Biology 14; Article number: 213; Dean, C., et al. (2003). Nat. Neurosci. 6(7); 708-716).

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.