Anti-Claudin-5 Antibody, clone 3H12 ZooMAb^® Rabbit Monoclonal

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ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Clone 3H12 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Claudin-5. It targets an epitope within 18 amino acids from the C-terminal, cytoplasmic domain.

KLH-conjugated linear peptide corresponding to 18 amino acids from the C-terminal, cytoplasmic domain of human Claudin-5.

Quality Control Testing

Evaluated by Western Blotting in MCF-7 cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Claudin-5 in MCF-7 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Claudin-5 in Human colon tissue lysates.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Claudin-5 in MCF-7 cells.

Affinity Binding Assay: A representative lot of this antibody bound Claudin-5 peptide with a KD of 3.7 x 10-8 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Claudin-5 in Human kidney tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Claudin-5 (UniProt: O00501; also known as Transmembrane protein deleted in VCFS, TMDVCF) is encoded by the CLDN5 (also known as AWAL, TMVCF) gene (Gene ID: 7122) in human. Claudin-5 is a multi-pass membrane protein with three cytoplasmic domains (aa 1-7; 103-122; and 181-218); four transmembrane domains (aa 8-28, 82-102, 123-143, and 160-180); and two extracellular loops (aa 29-81 and 144-159). It plays a major role in tight junction-specific obliteration of the intercellular space. It is localized specifically to the endothelial cell layer in the brain, lung, liver, kidney, and skin. In peripheral tissues, it displays greater heterogeneity in tissue expression. At the blood-brain barrier, it is one of the most enriched tight junction proteins and its dysfunction has been linked to several neurodegenerative diseases, including Alzheimer s disease and multiple sclerosis. Cldn5-null mice are reported to die shortly after birth. This ZooMAb^® recombinant monoclonal antibody, generated by

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.