Anti-TGF β receptor 1 Antibody, clone 4H18 ZooMAb^® Rabbit Monoclonal

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ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Clone 4H18 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects TGF-β receptor type 1 (TGF β-R1). It targets an epitope within 16 amino acids from the extracellular domain.

KLH-conjugated linear peptide corresponding to 16 amino acids from the extracellular domain of human TGF-β receptor type-1 (TGF β-R1).

Quality Control Testing

Evaluated by Western Blotting with IgG-His-tagged recombinant protein fragment from the extracellular domain of Human TGFβ receptor 1.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected IgG-His-tagged recombinant fragment corresponding to 100 amino acids from the extracellular domain of Human TGFβ receptor 1.

Tested Applications

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected TGF β receptor 1 in HepG2 cells.

Affinity Binding Assay: A representative lot of this antibody bound TGF β receptor 1 peptide with a KD of 1.2 x 10-7 in an affinity binding assay.

Enzyme Immunoassay (ELISA) Analysis: Serial dilutions from a representative lot detected hIgG-His-tagged recombinant fragment corresponding to 100 amino acids from the extracellular domain of Human TGF β receptor 1.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

TGF-beta receptor type-1 (UniProt: P36897; also known as EC:2.7.11.30, TGFR-1, Activin A receptor type II-like protein kinase of 53kD, Activin receptor-like kinase 5, ALK-5, Serine/threonine-protein kinase receptor R4, SKR4, Transforming growth factor-beta receptor type I, TbetaR-I) is encoded by the TGFBR1 (also known as ALK5, SKR4) gene (Gene ID: 7046) in human. TGF-β receptor type-1 is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-33), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 34-126), a transmembrane domain (aa 127-147), and a cytoplasmic domain (aa 148-503). It is widely expressed and is most abundant in placenta and least abundant in brain and heart. It is also expressed in a variety of cancer cell lines. TGF-β signaling involves its binding to the TGF-β receptor type II (TGF-β RII), which allows it to recruit TGF-β receptor type I (TGF-β RI) and assemble it as a heterod

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.