Anti-Syntaxin 4 Antibody, clone 1B9 ZooMAb^® Rabbit Monoclonal

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Clone 1B9 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Syntaxin 4. It targets an epitope within 19 amino acids from the C-terminal half.

KLH-conjugated linear peptide corresponding to 19 amino acids from the C-terminal half of human Syntaxin 4.

Quality Control Testing

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Syntaxin 4 in A431 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Syntaxin 4 in lysates from NIH3T3, and C6 cells and and Human kidney tissue.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Syntaxin 4 in Human kidney and Human prostate tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Syntaxin 4 in C6 cells.

Affinity Binding Assay: A representative lot of this antibody bound Syntaxin 4 peptide with a KD of 3.7 x 10-7 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Syntaxin-4 (UniProt: Q12846; also known as Renal carcinoma antigen NY-REN-31) is encoded by the STX4 (also known as STX4A) gene (Gene ID: 6810) in human. Syntaxins are a family of membrane proteins involved in vesicle trafficking. Syntaxin 4 (STX4) is single-pass type IV membrane protein with a single transmembrane domain (aa 276-296). It is a component of the SNARE complex that is composed of STX4, SNAP23 and VAMP7 that interacts with SYT7 during lysosomal exocytosis. In neuronal cells it localizes to neurite tips. It is recruited at neurite tips to membrane domains rich in the phospholipid 1-oleoyl-2-palmitoyl-PC that promotes neurite tip surface expression of the dopamine transporter SLC6A3/DAT by facilitating fusion of SLC6A3-containing transport vesicles with the plasma membrane. STX4 is reported to mediate retrograde signaling, modulating the membrane levels of synaptotagmin 4 and the trans-synaptic adhesion protein Neuroligin 1. STX4-dependent trafficking regulates synaptic deve

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.