Anti-DICER1 Antibody, clone 1E4 ZooMAb^® Rabbit Monoclonal

Helix is a trademark of Glass Expansion Pty Ltd

LONG is a registered trademark of Repligen Sweden AB

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Affinity is a trademark of Mine Safety Appliances Co.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Endoribonuclease Dicer (UniProt: Q9UPY3; also known as EC:3.1.26.3, Helicase with RNase motif, Helicase MOI) is encoded by the DICER1 (also known as DICER, HERNA, KIAA0928) gene (Gene ID: 23405) in human. DICER is a multidomain endonuclease that belongs to the fourth class of type III ribonuclease (RNase III) enzymes. It is a component of the RNA-induced silencing complex (RISC) that is composed of DICER1, AGO2 and TARBP2. Here DICER1 and TARBP2 are shown to be essential in processing precursor microRNAs (miRNA) into mature miRNAs and then load them onto AGO2. The processed mature RNAs are subsequently incorporated into RISC. DICER cleaves naturally occurring LONG^® dsRNAs and short hairpin pre-miRNA into fragments of twenty-one to twenty-three nucleotides with 3′ overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs that serve as a guide to direct RISC to complementary RNAs to degrade them or prevent their translation. DICER expression is tightly regulated and its inactivation results in loss of mature miRNAs and accumulation of miRNA precursors. Its dysfunctions and abnormal miRNA processing have been linked to aging and various neurodegenerative diseases. Its levels are downregulated in patients suffering from age-related diseases, such as Parkinson′s disease and amyotrophic lateral sclerosis (ALS). Mutations in DICER1 gene have been linked to Pleuropulmonary blastoma, a rare pediatric intrathoracic neoplasm that arises during fetal lung development. DICER1 consists of an N-terminal helicase, a platform-PAZ-connector Helix^™ domain (aa 891-1042), two RNASE III domains (aa 1276-1403 and 1666-1824) and a C-terminal double-stranded RNA-binding domain (aa 1849-1914). Three isoforms of DICER1 have been described that are produced by alternative splicing. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, Affinity^™, reproducibility, and stability over conventional monoclonals. (Ref.: Vergani-Junior, CA., et al. (2021). Biophys, Rev. 13(6); 1081-1090; Liu, Z., et al. (2018). Cell. 173(5); 1191-1203).

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

KLH-conjugated linear peptide corresponding to 20 amino acids from the internal region of human endoribonuclease Dicer (DICER1).

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.

Quality Control Testing

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected DICER1 in HeLa cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected DICER1 in A431 cell lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected DICER1 in Human small intestine tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound DICER1 peptide with a KD of 5.0 x 10-7 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 1E4 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects DICER1. It targets an epitope within 20 amino acids from the internal region.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.