Anti-TRIM8 Antibody, clone 1I18 ZooMAb^® Rabbit Monoclonal

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ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Clone 1I18 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects E3 ubiquitin-protein ligase TRIM8. It targets an epitope within 24 amino acids from the C-terminal region.

KLH-conjugated linear peptide corresponding to 24 amino acids from the C-terminal region of human E3 ubiquitin-protein ligase TRIM8.

Quality Control Testing

Evaluated by Western Blotting in L6 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected TRIM8 in L6 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected GST-tagged, full-length, recombinant Human TRIM8..

Affinity Binding Assay: A representative lot of this antibody bound TRIM8 peptide with a KD of 1.0 x 10-12 in an affinity binding assay.

Enzyme Immunoassay (ELISA) Analysis: A serial of dilutions from a representative lot detected GST-tagged, full-length, recombinant Human TRIM8..

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected TRIM8 in Human kidney tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

E3 ubiquitin-protein ligase TRIM8 (UniProt: Q9BZR9; also known as EC:2.3.2.27, Glioblastoma-expressed RING finger protein, RING finger protein 27, RING-type E3 ubiquitin transferase TRIM8, Tripartite motif-containing protein 8) is encoded by the TRIM8 (also known as GERP, RNF27) gene (Gene ID: 81603) in human. TRIM8 belongs to this TRIM family of proteins that are known to have a tripartite motif. It is widely expressed, homodimeric, protein that contains a ring domain (aa 15-36), two B box type domains (aa 92-132 and 140-180), a coiled-coil domain (aa 181-249), and a proline-rich no-domain C-terminal region (aa 351-551) with a monopartite nuclear localization signal (aa 373-383). The RING domain is shown to be crucial for its activity and regulates stabilization and activation of TP53, degradation of MDM2, and destabilization of Np63 . The conserved B-box and coiled-coil domain region is known to mediate the interaction with SOCS1, a tumor suppressor gene. The coiled-coil domain is

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.