Anti-MRGPRX2 Antibody, clone 1O10 ZooMAb^® Rabbit Monoclonal

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Clone 1O10 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Mas-related G-protein coupled receptor member X2 (MRGPRX2). It targets an epitope within 20 amino acids from the N-terminal, extracellular domain.

KLH-conjugated linear peptide corresponding to 20 amino acids from the N-terminal region of human Mas-related G-protein coupled receptor member X2 (MRGPRX2).

Quality Control Testing

Evaluated by Western Blotting in Human liver tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected MRGPRX2 in Human liver tissue lysate.

Tested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected MRGPRX2 in Human dorsal root ganglion tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound MRGPRX2 peptide with a KD of 3.4 x 10-6 in an affinity binding assay.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MRGPRX2 in MCF-7 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Mas-related G-protein coupled receptor member X2 (UniProt: Q96LB1; also known as MRGPRX2) is encoded by the MRGPRX2 (also known as MRGX2) gene (Gene ID: 117194) in human. MRGPRX2 is a multi-pass membrane protein with four extracellular domains, seven transmembrane domains, and four cytosolic domains. It is mainly expressed in mast cells; however, it has limited expression in peripheral and central nervous system, higher levels reported in small sensory cells in the dorsal root ganglion. It serves as a mast cell-specific receptor for basic secretagogues drug and for endogenous and exogenous peptides. Cortistain that has role in sleep regulation, locomotor activity, and cortical function has been recognized a high potency ligand for MRGPRX2. MRGPRX2 has also been linked to pseudo-allergic drug reactions to certain drugs, such as ciprofloxacin, and atracurium. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Navinés-Ferrer, A., et al. (2018). Sci Rep. 8; 11628; McNeil, BD., et al. (2015). Nature. 519(7542); 237-241; Robas, N., et al. (2003). J. Biol. Chem. 278(45); 44400-44404).

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.