生物来源
rabbit
质量水平
重组
expressed in HEK 293 cells
偶联物
unconjugated
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
1L3, recombinant monoclonal
描述
1L3 Clone
产品线
ZooMAb® learn more
形式
lyophilized
分子量
calculated mol wt 97.21 kDa
observed mol wt ~105 kDa
纯化方式
using Protein A
种属反应性
monkey, human, mouse
种属反应性(根据同源性预测)
bovine, sheep, feline
包装
antibody small pack of 25 μL
环保替代产品特性
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
增强验证
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
技术
affinity binding assay: suitable
immunohistochemistry: suitable
western blot: suitable
同位素/亚型
IgG
表位序列
Internal
Protein ID登记号
UniProt登记号
环保替代产品分类
运输
ambient
储存温度
2-8°C
靶向翻译后修饰
unmodified
基因信息
human ... AGO1(26523)
一般描述
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
特异性
Clone 1L3 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Protein argonaute-1 (Ago 1). It targets an epitope within 21 amino acids from the internal region.
免疫原
KLH-conjugated linear peptide corresponding to 21 amino acids from the internal region of human Protein argonaute-1 (Ago 1).
应用
Quality Control Testing
Evaluated by Western Blotting in NIH3T3 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Ago 1 in NIH3T3 cell lysate.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Ago 1 in K562 and COS-7 cell lysates.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Ago 1 in human placenta tissue sections.
Affinity Binding Assay:: A representative lot of this antibody bound Ago1 peptide with a KD of 3.1 x 10-7 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in NIH3T3 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Ago 1 in NIH3T3 cell lysate.
Evaluated by Western Blotting in NIH3T3 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Ago 1 in NIH3T3 cell lysate.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Ago 1 in K562 and COS-7 cell lysates.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Ago 1 in human placenta tissue sections.
Affinity Binding Assay:: A representative lot of this antibody bound Ago1 peptide with a KD of 3.1 x 10-7 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in NIH3T3 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Ago 1 in NIH3T3 cell lysate.
目标描述
Protein argonaute-1 (UniProt: Q9UL18; also known as Argonaute1, hAgo1, Argonaute RISC catalytic component 1, Eukaryotic translation initiation factor 2C 1, eIF-2C 1, eIF2C 1, Putative RNA-binding protein Q99) I encoded by the AGO1 (also known as EIF2C1) gene (Gene ID: 26523) in human. The highly conserved Argonaute proteins play a critical role in the regulation of gene expression and orchestrate the establishment and the maintenance of cell identity throughout the entire life cycle. In humans, four functional Argonaute proteins (Ago 1-4) have been described that exhibit high structure similarity, but also have a few non-conserved amino acids in their functional domains. In contrast to Ago 2, which has two N-terminal motifs that are required for its full catalytic activity, Ago1 contains only one N-terminal motif and Ago 3 and 4 lack these motifs and are catalytically inactive. About 80% of Ago1 clusters are associated with cell-type-specific transcriptional enhancers. Ago1 is required for RNA-mediated gene silencing. It binds to short RNAs such as miRNA or siRNA and represses the translation of mRNAs which are complementary to them. Its PIWI domain is localized in amino acids 515 to 816. Ago1 lacks RNA cleavage activity due to the absence of the conserved histidine at position 805. Ago1 is shown to be enriched around transcription start sites and binds preferentially to active transcriptional enhancers. Its association with enhancers is mediated by RNAs that are transcribed from these enhancers. However, the interaction of Ago1 with enhancers does not appear to regulate transcription of the neighboring genes. This ZooMAbZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Müller, M., et al. (2020). Front. Cell Dev. Biol. 7; 360; Allo, M., et al. (2014). Proc. Natl. Acad. Sci. USA. 111(44); 15622-15629).
外形
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重悬
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
储存及稳定性
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
法律信息
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
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