Anti-MAD2L1 Antibody, clone 1E9 ZooMAb^® Rabbit Monoclonal

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ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Clone 1E9 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Mitotic spindle assembly checkpoint protein MAD2A (MAD2). It targets an epitope within 18 amino acids from the C-terminal region.

KLH-conjugated linear peptide corresponding to 18 amino acids from the C-terminal region of human Mitotic spindle assembly checkpoint protein MAD2A.

Quality Control Testing

Evaluated by Western Blotting in L6 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected MAD2L1 in L6 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected MAD2L1 in HeLa and A431 cell lysates.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected MAD2L1 in human ovarian cancer tissue sections.

Affinity Binding Assay Analysis: A representative lot of this antibody bound Mad2L1 peptide with a KD of 1.8 x 10-7 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Mitotic spindle assembly checkpoint protein MAD2A (UniProt: Q13257; also known as HsMAD2, Mitotic arrest deficient 2-like protein 1, MAD2-like protein 1, MAD2) is encoded by the MAD2L1 (also known as MAD2) gene (Gene ID: 4085) in human. MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. It exists as a monomer or homodimer. It is reported to exist either in an open and inactive (O-MAD2) conformation or in the closed, active (C-MAD2) conformation. In the closed conformation it forms a heterotetrameric complex with MAD1L1 at unattached kinetochores during prometaphase, the complex recruits open conformation molecules of MAD2L1 (O-MAD2) and then promotes the conversion of O-MAD2 to C-MAD2. MAD2L1 undergoes phosphorylation on multiple serine residues by NIMA related kinase 2 (NEK2). The level of phosphorylation varies during the cell cycle and is shown to be the highest during mitosis. Phosphorylation abolishes interaction with MAD1L1 and reduces interaction with CDC20. Two isoforms of MAD2L1 are described that are produced by alternative splicing. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Ji, W., et al. (2018). J. Biol. Chem. 293(2); 484-496; Mapelli, M., et al. (2007). Cell. 131(4); 730-743; Luo, X., et al. (2000). Nat. Struct. Biol. 7(3); 224-229).

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.