ZRB1936
Anti-RUNX1 Antibody, clone 1C18 ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
登录查看公司和协议定价
所有图片(8)
Runt-related transcription factor 1;Acute myeloid leukemia 1 protein;Core-binding factor subunit alpha-2;CBF-alpha-2, Oncogene AML-1;Polyomavirus enhancer-binding protein 2 alpha B subunit;PEA2-alpha B;PEBP2-alpha B;SL3-3 enhancer factor 1 alpha B subunit
推荐产品
生物来源
rabbit
质量水平
重组
expressed in HEK 293 cells
偶联物
unconjugated
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
1C18, recombinant monoclonal
描述
1C18 Clone
产品线
ZooMAb® learn more
形式
lyophilized
分子量
calculated mol wt 48.74 kDa
observed mol wt ~52 kDa
纯化方式
using Protein A
种属反应性
human, rat, mouse
包装
antibody small pack of 25 μL
环保替代产品特性
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
增强验证
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
技术
affinity binding assay: suitable
immunocytochemistry: suitable
western blot: suitable
同位素/亚型
IgG
表位序列
Internal
Protein ID登记号
UniProt登记号
环保替代产品分类
运输
ambient
储存温度
2-8°C
靶向翻译后修饰
unmodified
基因信息
human ... RUNX1(861)
一般描述
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
特异性
Clone 1C18 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects RUNX1/AML1. It targets an epitope within 22 amino acids from the internal region.
免疫原
KLH-conjugated linear peptide corresponding to 22 amino acids from the internal region of human Runt-related transcription factor 1 (RUNX1).
应用
Quality Control Testing
Evaluated by Western Blotting in RAW264.7 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected RUNX1 in RAW264.7 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected RUNX1 in Rat Thymus lysates.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected RUNX1 in Jurkat cells.
Affinity Binding Assay: A representative lot of this antibody bound RUNX1 peptide with a KD of 2.5 x 10-7 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in RAW264.7 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected RUNX1 in RAW264.7 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected RUNX1 in Rat Thymus lysates.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected RUNX1 in Jurkat cells.
Affinity Binding Assay: A representative lot of this antibody bound RUNX1 peptide with a KD of 2.5 x 10-7 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
目标描述
Runt-related transcription factor 1 (UniProt: Q01196; also known as Acute myeloid leukemia 1 protein, Core-binding factor subunit alpha-2, CBF-alpha-2, Oncogene AML-1, Polyomavirus enhancer-binding protein 2 alpha B subunit, PEA2-alpha B, PEBP2-alpha B, SL3-3 enhancer factor 1 alpha B subunit, SL3/AKV core-binding factor alpha B subunit) is encoded by the RUNX1 (also known as AML1, CBFA2) gene (Gene ID: 861) in human. RUNX members are shown to modulate the transcription of their target genes through recognizing the core consensus binding sequence 5′-TGTGGT-3′. RUNX1 is a core-binding transcription factor that is reported to be essential for the establishment of definitive hematopoiesis in vertebrates. It is expressed in all tissues except brain and heart. Its highest levels are observed in thymus, bone marrow, and peripheral blood. RUNX1 forms the heterodimeric complex core-binding factor (CBF) with CBFB and controls the expression of its target genes involved in hematopoietic differentiation, ribosome biogenesis, cell cycle regulation, and p53 and transforming growth factor β signaling pathways. RUNX1 binds DNA as a monomer through its Runt domain (aa 50-178) and its DNA-binding is increased by heterodimerization. RUNX1 is phosphorylated on serine 249 and 276 and threonine 273 by homeodomain interacting protein kinase 2 (HIPK2) when associated with CBFB and DNA. This phosphorylation promotes subsequent EP300 phosphorylation. RUNX1 gene is one of the most frequently mutated genes in a variety of hematological malignancies. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Sood, R., et al. (2017). Blood. 129(15); 2070-2082 Wee, H-J., et al. (2008). Blood. 112(9); 3777-3787).
外形
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重悬
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
储存及稳定性
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
法律信息
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门