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生物来源
rabbit
质量水平
重组
expressed in HEK 293 cells
偶联物
unconjugated
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
2H13, recombinant monoclonal
描述
recombinant, expressed in HEK 293 cells
产品线
ZooMAb® learn more
形式
lyophilized
分子量
calculated mol wt 40.04 kDa
observed mol wt ~48 kDa
纯化方式
using Protein A
种属反应性
mouse, human, rat
包装
antibody small pack of 25 μL
环保替代产品特性
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
增强验证
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
技术
ELISA: suitable
affinity binding assay: suitable
immunocytochemistry: suitable
western blot: suitable
同位素/亚型
IgG
表位序列
N-terminal
Protein ID登记号
UniProt登记号
环保替代产品分类
运输
ambient
储存温度
2-8°C
靶向翻译后修饰
unmodified
基因信息
human ... PACC1(55248)
一般描述
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
特异性
Clone 2H13 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Transmembrane protein 206 (TMEM206). It targets an epitope within 24 amino acids from the N-terminal region.
免疫原
KLH-conjugated linear peptide corresponding to 24 amino acids from the N-terminal region of human Transmembrane protein 206 (TMEM206).
应用
Quality Control Testing
Evaluated by Western Blotting in Human brain tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected TMEM206 in Human brain tissue lysate.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected TMEM206 in lysates from Mouse brain tissue and PC12 cells, and recombinant Human TMEM206 protein.
Enzyme Immunoassay Analysis (ELISA): Serial dilutions from a representative lot detected TMEM206 protein.
Affinity Binding Assay: A representative lot of this antibody bound TMEM206 peptide with a KD of 1.0 x 10-12 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected TMEM206 in HeLa, A431, and NIH 3T3 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in Human brain tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected TMEM206 in Human brain tissue lysate.
Evaluated by Western Blotting in Human brain tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected TMEM206 in Human brain tissue lysate.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected TMEM206 in lysates from Mouse brain tissue and PC12 cells, and recombinant Human TMEM206 protein.
Enzyme Immunoassay Analysis (ELISA): Serial dilutions from a representative lot detected TMEM206 protein.
Affinity Binding Assay: A representative lot of this antibody bound TMEM206 peptide with a KD of 1.0 x 10-12 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected TMEM206 in HeLa, A431, and NIH 3T3 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in Human brain tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected TMEM206 in Human brain tissue lysate.
目标描述
Proton-activated chloride channel (UniProt: Q9H813; also known as PAC, Transmembrane protein 206) is encoded by the PACC1 (also known as TMEM206) gene (Gene ID: 55248) in human. Chloride ions (Cl ) are the most abundant anions in animal cells and their movement across cell membrane is mediated by Cl channels and transporters, which are essential for several cellular functions, such as regulation of cell volume, acidification of intracellular vesicles, and excitability control in muscle cell. TMEM206 is an evolutionarily conserved, multi-pass membrane glycoprotein with two cytoplasmic domains, two transmembrane domains, and one extracellular domain. It is widely expressed, but its highest expression is observed in the brain. It is an essential component of the acid-sensitive outwardly rectifying (ASOR) anion channel that is involved in import of chloride ions in response to extracellular acidic pH. It is also involved in acidosis-induced cell death by mediating chloride influx and subsequent cell swelling. TMEM206 -/- cells exhibit partial protection from acid-induced cell death. Higher expression of TMEM206 has been observed in colorectal cancer (CRC), breast, and liver cancers. Overexpression of TMEM206 promotes proliferation and invasion in CRC cells and up-regulates the levels of phospho-AKT and its downstream signaling components. This ZooMAbZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Deng, Z., et al. (2021). Science Adv. 7(9); eabe5983; Zhang, L., et al. (2020). Oncol. Lett. 5; 174; Ullrich, F., et al. (2019). eLife. 8; e49187).
外形
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重悬
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
储存及稳定性
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
法律信息
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
法规信息
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