Anti-Raptor Antibody, clone 3F15 ZooMAb^® Rabbit Monoclonal

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ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Clone 3F15 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Raptor. It targets an epitope within 22 amino acids from the N-terminal region.

KLH-conjugated linear peptide corresponding to 22 amino acids from the N-terminal region of human Raptor.

Quality Control Testing

Evaluated by Western Blotting in A549 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Raptor in A549 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Raptor in L6 cell lysate.

Affinity Binding Assay: A representative lot of this antibody bound Raptor peptide with a KD of 5.7 x 10-6 in an affinity binding assay.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Raptor in A431 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Regulatory-associated protein of mTOR (UniProt: Q8N122; also known as Raptor, p150 target of rapamycin (TOR)-scaffold protein) is encoded by the RPTOR (also known as KIAA1303, RAPTOR) gene (Gene ID: 57521) in human. Cellular mTOR is composed of two large protein complexes, known as mTORC1 and mTORC2. The mTORC1 complex is sensitive to rapamycin and consists of mTOR, Raptor, and mLST8 and two negative regulators, PRAS40 and DEPTOR. The Raptor unit serves as a scaffolding protein to bridge the interaction between mTOR and its substrates and contains seven WD repeats in its C-terminal half.  AMPK is reported to inhibit mTORC1 by directly phosphorylating Raptor on serine 722 and 792. Three isoforms of Raptor have been described that are produced by alternative splicing. Isoform 1 is highly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney, and placenta. Isoform 2 is a truncated for that lacks amino acids 380-1335. Isoform 3 is widely expressed, with highest levels in nasal mucosa and pituitary and lowest in spleen. Raptor is essential for mTORC1 activity and is critical for the regulation of mTORC1 activity in response to insulin signaling and nutrient and energy sufficiency. In addition to its role in energy metabolism and autophagy, Raptor/mTORC1 pathway is reported to play a role in lymphocyte activation through promoting 4EBP1 and S6 phosphorylation. Inhibition of Raptor/mTORC1 signaling blocks the lymphocyte expansion during primary adaptive immune response and impairs the lymphocyte mediated cytotoxic response. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Li, K., et al. (2019). Front. Immunol. 10; 2953; Kim, DH., et al. (2002).  Cell. 110(2); 163-175; Hara, K., et al. (2002). Cell. 110(2); 177-189).

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.