Anti-AGK Antibody, clone 1F21 ZooMAb^® Rabbit Monoclonal

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ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Clone 1F21 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Acylglycerol kinase (AGK). It targets an epitope within 15 amino acids from the C-terminal region.

KLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal region of human Acylglycerol kinase (AGK).

Quality Control Testing

Evaluated by Western Blotting in U2OS cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected AGK in U2OS cell lysate.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected AGK in HeLa and K562 cell lysates.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected AGK in K562 and U2OS cells.

Affinity Binding Assay: A representative lot of this antibody bound AGK with a KD of 6.7 x 10-9 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected AGK in human heart tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Acylglycerol kinase, mitochondrial (UniProt: Q53H12; also known as EC:2.7.1.107, hAGK, Multiple substrate lipid kinase, HsMuLK, MuLK, Multi-substrate lipid kinase) is encoded by the AGK (also known as MULK) gene (Gene ID: 55750) in human. AGK is an inner mitochondrial membrane enzyme that is highly expressed in muscle, heart, kidney, and brain. It is a multi-substrate lipid kinase with a typical two-domain fold (DGK1 and 2) that can phosphorylate both monoacylglycerol and diacylglycerol to form lysophosphatidic acid (LPA) and phosphatidic acid (PA), respectively. It has an N-terminal 1 helix, which is complete across the membrane, and a C-terminal key region bound to the membrane. Independently of its lipid kinase activity, it also acts as a component of the TIM22 complex and plays a role in stabilizing the TIM22 complex and regulating the import and assembly of mitochondrial carrier proteins. Overexpression of AGK is reported to increase the formation and secretion of LPA, resulting in transactivation of EGFR and activation of the downstream MAPK signal transduction pathway that leads to increased cell growth. AGK has been identified as an oncogene and its overexpression of is observed in several tumor types, including prostate cancer, breast cancer, and cervical squamous cell carcinoma. This ZooMAbZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Chu, B., et al. (2021). Front. Cell Dev. Biol. 9; 659158; Vukotic, M., et al. (2017). Mol. Cell. 67(3); 471-483; Bektas, M., et al. (2005). J. Cell Biol. 169(5); 801-811).

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.