推荐产品
生物来源
rabbit
质量水平
重组
expressed in HEK 293 cells
偶联物
unconjugated
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
1O8, monoclonal
recombinant monoclonal
产品线
ZooMAb® learn more
形式
lyophilized
分子量
calculated mol wt ~28.32 and 20.66 kDa
observed mol wt ~30 and 18 kDa
种属反应性
human
包装
antibody small pack of 25 μL
环保替代产品特性
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
增强验证
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
技术
affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
同位素/亚型
IgG
UniProt登记号
环保替代产品分类
, Aligned
运输
ambient
储存温度
2-8°C
靶向翻译后修饰
unmodified
一般描述
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
特异性
Clone 1O8 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects human Methyltransferase-like protein 7A (METTL7A). It targets an epitope within 21 amino acids from the C-terminal region and detects both isoforms.
免疫原
KLH-conjugated linear peptide corresponding to 21 amino acids from the C-terminal region of human Methyltransferase-like protein 7A (METTL7A).
应用
Quality Control Testing
Evaluated by Western Blotting in HT-1376 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Methyltransferase-like protein 7A (METTL7A) in HT-1376 cell lysate.
Tested Applications
Affinity Binding Assay: A representative lot of this antibody bound METTL7A peptide with a KD of 1.1 x 10-7 in an affinity binding assay.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected METTL7A in human testis tissue sections.
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected METTL7A in HepG2 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in HT-1376 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Methyltransferase-like protein 7A (METTL7A) in HT-1376 cell lysate.
Tested Applications
Affinity Binding Assay: A representative lot of this antibody bound METTL7A peptide with a KD of 1.1 x 10-7 in an affinity binding assay.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected METTL7A in human testis tissue sections.
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected METTL7A in HepG2 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-METTL7A, clone 1O8 ZooMAb®, Cat. No. ZRB1185, is a rabbit recombinant monoclonal antibody that targets METTL7A and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
目标描述
Methyltransferase-like protein 7A (UniProt: Q9H8H3 and computationally mapped F8VQX6, Q9H8H3, also known as AAM-B, METTL7A) is encoded by the METTL7A gene (Gene ID: 25840) in human. METTL7A is a ubiquitously expressed lipid droplets-associated protein that displays high expression in liver and thyroid tissues. It is synthesized with a signal peptide (aa 1-29) and is an integral membrane protein anchored into the endoplasmic reticulum (ER) membrane. It contains N and C-terminal domains, juxtamembrane region, and a putative central catalytic domain for methyltransferase activity. The N-terminal regions contains a hydrophobic sequence of 28 amino acids that is shown to be essential for its insertion into ER. It also helps recruit other cellular protein to lipid droplets for the formation of functional organelles. METTL7A is reported to be essential for the assembly and release stages of hepatitis C (HCV) life cycle but is not essential for the replication step of virus. Knockdown of METTL7A is shown to impair HCV propagation. METTL7A gene is reported to be downregulated in thyroid cancer. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Zhou, S., et al., (2017). Oncotarget. 8(21); 34652-34660; Park, EM., et al. (2015). PLoS One. 10(7); e0132839).
外形
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重悬
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
储存及稳定性
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
法律信息
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
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