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生物来源
rabbit
质量水平
重组
expressed in HEK 293 cells
偶联物
unconjugated
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
2E6, monoclonal
recombinant monoclonal
产品线
ZooMAb® learn more
形式
lyophilized
分子量
calculated mol wt ~57.42 kDa
observed mol wt ~64 kDa
种属反应性
human
种属反应性(根据同源性预测)
mouse, rat, monkey, bovine
包装
antibody small pack of 25 μL
环保替代产品特性
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
增强验证
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
技术
affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
同位素/亚型
IgG
UniProt登记号
环保替代产品分类
, Aligned
运输
ambient
储存温度
2-8°C
靶向翻译后修饰
unmodified
一般描述
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
特异性
Clone 2E6 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects vesicular GABA transporter. It targets an epitope within 15 amino acids from the C-terminal, cytoplasmic domain.
免疫原
KLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal, cytoplasmic domain of human Vesicular GABA transporter.
应用
Quality Control Testing
Evaluated by Western Blotting in Human Brain tissue lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected VGAT in Human Brain tissue lysate.
Tested Applications
Immunocytochemistry Analysis: A 1:100 Dilution from a representative lot detected VGAT in Y79 cell line.
Affinity Binding Assay Analysis: A representative lot of this antibody bound VGAT peptides with a KD of 5.3 x 10^-8 in an affinity binding assay.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected VGAT in human cerebellum tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in Human Brain tissue lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected VGAT in Human Brain tissue lysate.
Tested Applications
Immunocytochemistry Analysis: A 1:100 Dilution from a representative lot detected VGAT in Y79 cell line.
Affinity Binding Assay Analysis: A representative lot of this antibody bound VGAT peptides with a KD of 5.3 x 10^-8 in an affinity binding assay.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected VGAT in human cerebellum tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-VGAT, clone 2E6 ZooMAb®, Cat. No. ZRB1158, is a recombinant Rabbit monoclonal antibody that specifically targets VGAT and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
目标描述
Vesicular inhibitory amino acid transporter (UniProt: Q9H598; also known as GABA and glycine transporter, Solute carrier family 32 member 1, Vesicular GABA transporter, hVIAAT, VGAT) is encoded by the SLC32A1 (also known as VGAT, VIAAT) gene (Gene ID: 140679) in human. VGAT is a multi-pass membrane protein with six cytoplasmic domains, ten transmembrane domains, and five vesicular lumenal domains. It is shown to be highly concentrated in the nerve endings of GABAergic neurons and in glycinergic nerve endings. It transports GABA and glycine into acidic vesicles and localizes to the synaptic vesicle in glycinergic and GABAergic neurons. It is also expressed throughout the horizontal cell tips at photoreceptor terminals in retina. VGAT expression has been reported in pancreatic islets of rat, however, its expression in human pancreatic islets is none to very low. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Chessler, SD., et al. (2002). Diabetes. 51(6); 1763-1771).
外形
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重悬
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
储存及稳定性
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
法律信息
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
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