Anti-LILRB4 Antibody, clone 1H12 ZooMAb^® Rabbit Monoclonal

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ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Clone 1H12 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects human Leukocyte immunoglobulin-like receptor subfamily B member 4 (LILRB4). It targets an epitope within 19 amino acids from the extracellular domain.

KLH-conjugated linear peptide corresponding to 19 amino acids from the extracellular domain of human Leukocyte immunoglobulin-like receptor subfamily B member 4 (LILRB4).

Quality Control Testing

Evaluated by Western Blotting in MDA-MB-435 lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected LILRB4 in MDA-MB-435 cell lysate.

Tested Applications

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected LILRB4 in NK-92 cells.

Affinity Binding Assay: A representative lot of this antibody bound LILRB4 peptide with a KD of 2.5 x 10-7 in an affinity binding assay.

Flow Cytometry Analysis: 1 μg from a representative lot detected LILRB4 in NK-92 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-LILRB4, clone 1H12 ZooMAb^®, Cat. No. ZRB1113, is a rabbit recombinant monoclonal antibody that specifically targets LILRB4 and is tested for use in Affinity Binding Assay, Flow Cytometry, Immunocytochemistry, and Western Blotting.

Leukocyte immunoglobulin-like receptor subfamily B member 4 (UniProt: Q8NHJ6; also known as CD85 antigen-like family member K, Immunoglobulin-like transcript 3, ILT-3, Leukocyte immunoglobulin-like receptor 5, LIR-5, Monocyte inhibitory receptor HM18, CD85k, LILRB4) is encoded by the LILRB4 (Gene ID: ILT3, LIR5) gene (Gene ID: 11006) in human. LILRB4 is a single-pass type I membrane protein that serves as a receptor for class I MHC antigens and recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. It is involved in the downregulation of the immune response and the development of tolerance, such as towards transplants. It is reported to inhibit receptor-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions. LILRB4 expression has been reported in monocytes, macrophages, dendritic cells, lung, natural killer cells and B-cells and is induced upon contact with CD8+ CD28- alloantigen-specific T suppressor (Ts) cells. LILRB4 is also a tumor-associated antigen that is highly expressed on monocytic AML cells where its expression is more specific and can be used to differentiate monocytic AML (M5) from AML M1-M3. It is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 22-259), a transmembrane domain (aa 260-280), and a cytoplasmic domain (aa 181-448). It contains three copies of immunoreceptor tyrosine-based inhibitor motif (ITIM) that are involved in modulation of cellular responses and the phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: John, S. et al. (2018). Mol. Therap. 26(10); 2487-2495).

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.