Anti-FTO Antibody, clone 4B21 ZooMAb^® Rabbit Monoclonal

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ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

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Clone 4B21 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Fat mass and obesity-associated protein (FTO). It targets and epitope within 20 amino acids from the N-terminal region.

KLH-conjugated linear peptide corresponding to 20 amino acids from the N-terminal region of human Fat mass and obesity-associated protein (FTO).

Anti-FTO, clone 4B21 ZooMAb, Cat. No. ZRB1052, is a recombinant Rabbit monoclonal antibody that specifically targets FTO and is tested in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected FTO in HepG2 and COS-7 cell lysates.

Immunocytochemistry Analysis: 1:100 from a representative lot detected FTO in HeLa, A431, HepG2 and NIH 3T3 cells.

Affinity Binding Assay: A representative lot of this antibody bound FTO peptide with a KD of 4.1 x 10-8 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected FTO in human testis and human kidney tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Alpha-ketoglutarate-dependent dioxygenase FTO (UniProt: Q9C0B1; also known as Fat mass and obesity-associated protein, U6 small nuclear RNA (2′-O-methyladenosine-N(6)-)-demethylase FTO, U6 small nuclear RNA N(6)-methyladenosine-demethylase FTO, mRNA (2′-O-methyladenosine-N(6)-)-demethylase FTO, m6A(m)-demethylase FTO, mRNA N(6)-methyladenosine demethylase FTO, tRNA N1-methyl adenine demethylase FTO) is encoded by the FTO (also known as KIAA1752) gene (Gene ID: 79068) in human. FTO is a ubiquitously expressed protein, but relatively higher expression is observed in adrenal glands and brain; especially in hypothalamus and pituitary. It is mainly localized in the nucleus where it serves as an RNA demethylase and mediates oxidative demethylation of different RNA species and acts as a regulator of fat mass, adipogenesis and energy homeostasis. It is shown to specifically demethylate N6-methyladenosine (m6A) RNA, which is one of the most prevalent internal modification of messenger RNA in higher eukaryotes. FTO can also act as a tRNA demethylase by removing N1-methyladenine from various tRNAs. However, it does not display any activity towards 1-methylguanine and double-stranded DNA. FTO is involved in the regulation of thermogenesis, fat mass, adipogenesis and body weight, thereby contributes to the regulation of body size and body fat accumulation. It is also reported to control adipocyte differentiation into brown or white fat cells. Its activity is enhanced in the presence of ascorbate and inhibited by N-oxalylglycine, fumarate and succinate. Mutations in FTO gene or altered expression of adjacent genes, such as IRX3, have been linked to obesity. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.