跳转至内容
Merck
CN
所有图片(15)

文件

安全信息

ZMS1137

Sigma-Aldrich

Anti-Tau Antibody, clone T49 ZooMAb® Mouse Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

登录查看公司和协议定价

别名:
Microtubule-associated protein tau;Neurofibrillary tangle protein;Paired helical filament-tau;PHF-tau
UNSPSC代码:
12352203
NACRES:
NA.41

生物来源

mouse

质量水平

重组

expressed in HEK 293 cells

偶联物

unconjugated

抗体形式

purified antibody

抗体产品类型

primary antibodies

克隆

T49, recombinant monoclonal

产品线

ZooMAb® learn more

形式

lyophilized

分子量

calculated mol wt 46.33 kDa
observed mol wt ~53 to 57 kDa

纯化方式

using protein G

种属反应性

rat, bovine, mouse

包装

antibody small pack of 25 μL

环保替代产品特性

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

增强验证

recombinant expression
Learn more about Antibody Enhanced Validation

sustainability

Greener Alternative Product

技术

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

同位素/亚型

IgG1κ

表位序列

Unknown

Protein ID登记号

UniProt登记号

环保替代产品分类

运输

ambient

储存温度

2-8°C

靶向翻译后修饰

unmodified

基因信息

bovine ... MAPT(281296)

一般描述

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

特异性

Clone T49 is a ZooMAb® Mouse recombinant monoclonal antibody that specifically detects Tau protein.

免疫原

Full-length purified Bovine Tau protein.

应用

Quality Control Testing

Evaluated by Western Blotting in Bovine brain tissue membrane preparations.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Tau in Bovine brain membrane preparation.

Tested Applications


Immunohistochemistry (Paraffin) Analysis: A 1:100-1,000 dilution from a representative lot detected Tau in Rat brain, Mouse brain. and Mouse kidney tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Tau in Neuro-2a cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

目标描述

Microtubule-associated protein tau (UniProt: P29172; also known as Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau) is encoded by the MAPT (also known as Tau) gene (Gene ID: 281296) in bovine species. Microtubule-associated protein tau that is expressed in neurons  is mostly found in axons, in the cytosol, and in association with plasma membrane components. Twenty isoforms of bovine Tau have been described that are generated by alternative splicing. Tau proteins promote microtubule assembly and stability and may also be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, thus serving as a linker protein between both. The short isoforms are reported to allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization. In Alzheimer disease (AD), the neuronal cytoskeleton in the brain is progressively disrupted and replaced by tangles of paired helical filaments (PHF) and straight filaments, mainly composed of hyperphosphorylated forms of Tau. In AD brain  Tau undergoes phosphorylation at various serine and threonine residues in S-P or T-P motifs by proline-directed protein kinases and at serine residues in K-X-G-S motifs by MAP and microtubule affinity-regulating kinases (MARK1, MARK2, MARK3, MARK4) that results in detachment from microtubules, and their disassembly. It also undergoes O-glycosylation at least on four GlcNAc and site-specific or stoichiometric changes in glycosylation may modulate tau function and also play a role in PHF′s formation. Tau is shown to accumulate in both the somatodendritic and axonal regions of neurons and in the soma as neurofibrillary tangles (NFTs). Tau oligomers are reported to be neurotoxic when applied extracellularly to cultured neuronal cells and can induce neurodegeneration and synaptic and mitochondrial dysfunction in vivo. It has been reported that most of the C-terminus of Tau in PHFs can be cleaved by trypsin, however the TauC4 region is highly resistant to trypsin action. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Taniguchi Watanabe, S., et al. (2016). Acta Neuropathol. 131(2); 267-280; Ando K., et al. (2010). Biochem. Soc. Trans. 38(4); 1001-1005; Arnold, CS., et al. (1996). J. Biol. Chem. 271(46); 28741-28744).

外形

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

重悬

0.3 mg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

新产品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门