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生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
purified from hybridoma cell culture
抗体产品类型
primary antibodies
克隆
PYP-76, monoclonal
形式
buffered aqueous solution
分子量
antigen ~65 kDa
种属反应性
human
技术
indirect ELISA: suitable
indirect immunofluorescence: suitable
western blot: 0.5-1 μg/mL using total cell extract of HEK-293T cells co-transfected with wt YAP and a constitutively active mutant of c-Abl (Δ1-81)
同位素/亚型
IgM
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
phosphorylation (pTyr357)
基因信息
human ... YAP1(10413)
mouse ... Yap1(22601)
一般描述
Monoclonal Anti-phospho-YAP1 (pTyr357) (mouse IgM isotype) is derived from the hybridoma PYP-76 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic phosphopeptide corresponding to a fragment containing (pTyr357) of human YAP1 conjugated to KLH. Yes-associated protein 1 (YAP1) is a modular adapter protein with multiple protein interaction domains that was originally identified based on its interaction with the Src-family tyrosine kinase c-Yes. YAP1 contains a proline-rich amino terminus, a WW domain, a coiled-coil and a PDZ-binding motif at the extreme C-terminus.
应用
Anti-phospho-YAP1 (pTyr357) antibody, Mouse monoclonal has been used in
- immunoblotting
- enzyme linked immunosorbent assay (ELISA)
- immunofluorescence
生化/生理作用
YAP1 has been shown to stabilize p73 by preventing Itch-mediated ubiquitination of p73.
YAP1 is a WW domain containing protein that is known to associate with a wide range of transcription factors and functions as a transcriptional co-activator. It may also regulate p73 gene function in response to DNA damage. Alterations in YAP1 have been associated with tumor development
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
Ting-Fang Lee et al.
Oncotarget, 8(52), 89539-89551 (2017-11-23)
Epidermal growth factor receptor (EGFR) mutations are found in lung adenocarcinomas leading to tumor cells proliferation and survival. EGFR tyrosine kinase inhibitors (TKIs) that block EGFR activity are effective therapeutics for EGFR-mutant lung adenocarcinoma patients, but TKI-resistance inevitably occurs. The
Conjugated Bile Acids Promote Invasive Growth of Esophageal Adenocarcinoma Cells and Cancer Stem Cell Expansion via Sphingosine 1-Phosphate Receptor 2-Mediated Yes-Associated Protein Activation
Liu R, et al.
The American Journal of Pathology, 188(9), 2042-2058 (2018)
Fernando D Camargo et al.
Current biology : CB, 17(23), 2054-2060 (2007-11-06)
The mechanisms that regulate mammalian organ size are poorly understood. It is unclear whether the pathways that control organ size also impinge on stem/progenitor cells. A highly expressed gene in stem cells is YAP1, the ortholog of Drosophila Yorkie, a
The Yes-associated protein 1 stabilizes p73 by preventing Itch-mediated ubiquitination of p73
Levy D, et al.
Cell Death and Differentiation, 14(4), 743-743 (2007)
Akihiko Komuro et al.
The Journal of biological chemistry, 278(35), 33334-33341 (2003-06-17)
The ErbB-4 receptor protein-tyrosine kinase is proteolytically processed by membrane proteases in response to the ligand or 12-O-tetradecanoylphorbol-13-acetate stimulation resulting in the cytoplasmic fragment translocating to the cell nucleus. The WW domain-containing co-transcriptional activator Yes-associated protein (YAP) associates physically with
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